Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6145 | 3' | -48.7 | NC_001826.1 | + | 104562 | 0.66 | 0.997533 |
Target: 5'- --gGUGAugGGGGGUUUGGCGuUUGa -3' miRNA: 3'- cuaCAUUugUUCUCGAGCCGCuAGCc -5' |
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6145 | 3' | -48.7 | NC_001826.1 | + | 6738 | 0.66 | 0.997533 |
Target: 5'- uGGUGUG---GAGAGCUUGGCGccAUCu- -3' miRNA: 3'- -CUACAUuugUUCUCGAGCCGC--UAGcc -5' |
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6145 | 3' | -48.7 | NC_001826.1 | + | 102118 | 0.67 | 0.99589 |
Target: 5'- cGGUGggGAGgGGGAGgaCGGgGAUCGa -3' miRNA: 3'- -CUACa-UUUgUUCUCgaGCCgCUAGCc -5' |
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6145 | 3' | -48.7 | NC_001826.1 | + | 70719 | 0.67 | 0.99589 |
Target: 5'- gGGUGUggguucagGAGCGGGAGCUuUGGCGGg-GGu -3' miRNA: 3'- -CUACA--------UUUGUUCUCGA-GCCGCUagCC- -5' |
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6145 | 3' | -48.7 | NC_001826.1 | + | 54349 | 0.67 | 0.99589 |
Target: 5'- uGAUGUugcCAGGAGCUCuugaacuuggGGUGAUCu- -3' miRNA: 3'- -CUACAuuuGUUCUCGAG----------CCGCUAGcc -5' |
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6145 | 3' | -48.7 | NC_001826.1 | + | 58099 | 0.72 | 0.921531 |
Target: 5'- --gGUGAGCAAGGGCcacauacUGGUGAUUGGu -3' miRNA: 3'- cuaCAUUUGUUCUCGa------GCCGCUAGCC- -5' |
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6145 | 3' | -48.7 | NC_001826.1 | + | 101927 | 1.11 | 0.009702 |
Target: 5'- gGAUGUAAACAAGAGCUCGGCGAUCGGc -3' miRNA: 3'- -CUACAUUUGUUCUCGAGCCGCUAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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