Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6146 | 3' | -64.4 | NC_001826.1 | + | 5220 | 0.72 | 0.202396 |
Target: 5'- uGCGUGGCCGGGaagucugUCUCGCGGCgGUCu -3' miRNA: 3'- -CGCGCUGGCCCg------AGGGUGCCGaCGGc -5' |
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6146 | 3' | -64.4 | NC_001826.1 | + | 102077 | 1.1 | 0.000346 |
Target: 5'- cGCGCGACCGGGCUCCCACGGCUGCCGg -3' miRNA: 3'- -CGCGCUGGCCCGAGGGUGCCGACGGC- -5' |
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6146 | 3' | -64.4 | NC_001826.1 | + | 118920 | 0.67 | 0.435303 |
Target: 5'- gGCGCGG-CGGGC-CCCGguuccccccgcccCGGCccGCCGc -3' miRNA: 3'- -CGCGCUgGCCCGaGGGU-------------GCCGa-CGGC- -5' |
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6146 | 3' | -64.4 | NC_001826.1 | + | 119114 | 0.69 | 0.319334 |
Target: 5'- gGCGCGGCCgcuaccgcccGGGC-CugaggacgggCCGCGGCggGCCGg -3' miRNA: 3'- -CGCGCUGG----------CCCGaG----------GGUGCCGa-CGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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