Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6148 | 3' | -61.9 | NC_001826.1 | + | 99609 | 0.69 | 0.419152 |
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Target: 5'- gGGCCCCGgagcgaGGGAGcggGCUGCccGGCcCGGGg -3' miRNA: 3'- -CCGGGGC------UCCUCa--CGACG--CUGcGCCU- -5' |
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| 6148 | 3' | -61.9 | NC_001826.1 | + | 99709 | 0.69 | 0.419152 |
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Target: 5'- gGGCCCCGgagcgaGGGAGcggGCUGCccGGCcCGGGg -3' miRNA: 3'- -CCGGGGC------UCCUCa--CGACG--CUGcGCCU- -5' |
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| 6148 | 3' | -61.9 | NC_001826.1 | + | 100907 | 0.69 | 0.419152 |
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Target: 5'- gGGCCCCGgagcgaGGGAGcggGCUGCccGGCcCGGGg -3' miRNA: 3'- -CCGGGGC------UCCUCa--CGACG--CUGcGCCU- -5' |
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| 6148 | 3' | -61.9 | NC_001826.1 | + | 118597 | 0.7 | 0.36216 |
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Target: 5'- aGCCgCCGAGGGG-GCUGgGGuCGgGGGa -3' miRNA: 3'- cCGG-GGCUCCUCaCGACgCU-GCgCCU- -5' |
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| 6148 | 3' | -61.9 | NC_001826.1 | + | 118217 | 0.76 | 0.160291 |
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Target: 5'- cGGCCCagggcucgggagUGGGGGGUGCUGgGGgGUGGAg -3' miRNA: 3'- -CCGGG------------GCUCCUCACGACgCUgCGCCU- -5' |
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| 6148 | 3' | -61.9 | NC_001826.1 | + | 117903 | 1.1 | 0.000601 |
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Target: 5'- uGGCCCCGAGGAGUGCUGCGACGCGGAg -3' miRNA: 3'- -CCGGGGCUCCUCACGACGCUGCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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