Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
615 | 3' | -60.1 | AC_000017.1 | + | 8247 | 0.66 | 0.377177 |
Target: 5'- cGUCUGGCU-GCUCGAGGggaguuacgguggaUCGGACCa -3' miRNA: 3'- uCAGGCCGGcCGGGCUCUg-------------AGCUUGG- -5' |
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615 | 3' | -60.1 | AC_000017.1 | + | 12233 | 0.66 | 0.354163 |
Target: 5'- aGGUCCGGCCccagcugccuccagGGCgCGucGGCUUgGGGCCc -3' miRNA: 3'- -UCAGGCCGG--------------CCGgGCu-CUGAG-CUUGG- -5' |
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615 | 3' | -60.1 | AC_000017.1 | + | 12511 | 0.67 | 0.316516 |
Target: 5'- gAG-CCGGuuGGCCUGuGGCUgcuGCCg -3' miRNA: 3'- -UCaGGCCggCCGGGCuCUGAgcuUGG- -5' |
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615 | 3' | -60.1 | AC_000017.1 | + | 2282 | 0.68 | 0.300697 |
Target: 5'- gGGUCCaGGCCGGCUcuCGGGuuccaugggcucuGCUCcuGCCg -3' miRNA: 3'- -UCAGG-CCGGCCGG--GCUC-------------UGAGcuUGG- -5' |
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615 | 3' | -60.1 | AC_000017.1 | + | 21891 | 0.69 | 0.255622 |
Target: 5'- uAGUUaacaCGGCCGGUCgCGAGACUgGGgguguacacuggaugGCCu -3' miRNA: 3'- -UCAG----GCCGGCCGG-GCUCUGAgCU---------------UGG- -5' |
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615 | 3' | -60.1 | AC_000017.1 | + | 10239 | 0.69 | 0.246669 |
Target: 5'- cGGUCUGGuggcCCGGCUgCGAGAgCUCGGugUa -3' miRNA: 3'- -UCAGGCC----GGCCGG-GCUCU-GAGCUugG- -5' |
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615 | 3' | -60.1 | AC_000017.1 | + | 5910 | 0.69 | 0.240435 |
Target: 5'- -cUUCGuGCUGGCCUG-GACgCGAGCCu -3' miRNA: 3'- ucAGGC-CGGCCGGGCuCUGaGCUUGG- -5' |
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615 | 3' | -60.1 | AC_000017.1 | + | 25035 | 0.7 | 0.200408 |
Target: 5'- ---gCGcGCUGGCuuGAGACgcgCGAGCCu -3' miRNA: 3'- ucagGC-CGGCCGggCUCUGa--GCUUGG- -5' |
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615 | 3' | -60.1 | AC_000017.1 | + | 10915 | 0.75 | 0.090617 |
Target: 5'- gAGUCgCGGgacccCCGGUUCGAGuCUCGGGCCg -3' miRNA: 3'- -UCAG-GCC-----GGCCGGGCUCuGAGCUUGG- -5' |
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615 | 3' | -60.1 | AC_000017.1 | + | 10734 | 0.77 | 0.066341 |
Target: 5'- ---aCGGCCGGauaCGGGGCUCGAACCc -3' miRNA: 3'- ucagGCCGGCCgg-GCUCUGAGCUUGG- -5' |
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615 | 3' | -60.1 | AC_000017.1 | + | 10992 | 1.11 | 0.000148 |
Target: 5'- cAGUCCGGCCGGCCCGAGACUCGAACCg -3' miRNA: 3'- -UCAGGCCGGCCGGGCUCUGAGCUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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