Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
616 | 5' | -52.8 | AC_000017.1 | + | 25461 | 0.66 | 0.700981 |
Target: 5'- uUCAGGUUGc-GCUCcuccAgGCaCUGCUGCc -3' miRNA: 3'- -AGUCCAAUucCGAG----UgCGaGACGACG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 25102 | 0.67 | 0.670981 |
Target: 5'- gUCGGc---AGGCUCGCGCgUCUcaagccagcgcgccaGCUGCu -3' miRNA: 3'- -AGUCcaauUCCGAGUGCG-AGA---------------CGACG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 12761 | 0.67 | 0.643035 |
Target: 5'- cCAGGUUGcccuGCUgcugcgCGCGCUCacGCUGCg -3' miRNA: 3'- aGUCCAAUuc--CGA------GUGCGAGa-CGACG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 33249 | 0.67 | 0.62786 |
Target: 5'- aUCAGGaUAGGGCgguggugcugcagcaGCGCgcgaauaaaCUGCUGCc -3' miRNA: 3'- -AGUCCaAUUCCGag-------------UGCGa--------GACGACG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 26929 | 0.67 | 0.618524 |
Target: 5'- aCGGGUUcguuGGGCgccagacgcaGCGCUCcuccuccUGCUGCu -3' miRNA: 3'- aGUCCAAu---UCCGag--------UGCGAG-------ACGACG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 5238 | 0.68 | 0.550292 |
Target: 5'- gCGGGUUGGGGCg---GCUUUcGCUGUa -3' miRNA: 3'- aGUCCAAUUCCGagugCGAGA-CGACG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 10098 | 0.69 | 0.537792 |
Target: 5'- cCAGGUc--GGCgacaaCGCGCUCggcuaauauggccUGCUGCa -3' miRNA: 3'- aGUCCAauuCCGa----GUGCGAG-------------ACGACG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 5342 | 0.71 | 0.420778 |
Target: 5'- gUCAcGGUgAAGGggUGCGCUCcggGCUGCg -3' miRNA: 3'- -AGU-CCAaUUCCgaGUGCGAGa--CGACG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 2267 | 0.71 | 0.420778 |
Target: 5'- cUCGGGUUccauGGGCUC-UGCUcCUGCcGCc -3' miRNA: 3'- -AGUCCAAu---UCCGAGuGCGA-GACGaCG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 12419 | 0.72 | 0.363261 |
Target: 5'- -uGGaGUUAAGGCcggacggCugGCUCUGCaGCg -3' miRNA: 3'- agUC-CAAUUCCGa------GugCGAGACGaCG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 1312 | 0.73 | 0.303355 |
Target: 5'- gCAGGUUccGGUUCugGCUCggGCUcagGCu -3' miRNA: 3'- aGUCCAAuuCCGAGugCGAGa-CGA---CG- -5' |
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616 | 5' | -52.8 | AC_000017.1 | + | 13349 | 1.14 | 0.000406 |
Target: 5'- aUCAGGUUAAGGCUCACGCUCUGCUGCa -3' miRNA: 3'- -AGUCCAAUUCCGAGUGCGAGACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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