Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6160 | 5' | -50.6 | NC_001837.1 | + | 8903 | 0.66 | 0.522106 |
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Target: 5'- gGGACCaucuuGAUGUAUCCcUGgCACCc -3' miRNA: 3'- -CCUGGcuau-CUGCAUGGGaAUgGUGG- -5' |
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| 6160 | 5' | -50.6 | NC_001837.1 | + | 2781 | 0.66 | 0.484764 |
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Target: 5'- uGGCUGG-GGugGUgucuuggGCCCUUGUCACCa -3' miRNA: 3'- cCUGGCUaUCugCA-------UGGGAAUGGUGG- -5' |
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| 6160 | 5' | -50.6 | NC_001837.1 | + | 4312 | 0.66 | 0.474161 |
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Target: 5'- uGGGCaug-GGACGUGCCCggUUGCUGgCg -3' miRNA: 3'- -CCUGgcuaUCUGCAUGGG--AAUGGUgG- -5' |
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| 6160 | 5' | -50.6 | NC_001837.1 | + | 5636 | 0.67 | 0.439693 |
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Target: 5'- uGGACCGcgGGGgugcugGUGCCCccGgCACCa -3' miRNA: 3'- -CCUGGCuaUCUg-----CAUGGGaaUgGUGG- -5' |
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| 6160 | 5' | -50.6 | NC_001837.1 | + | 490 | 0.68 | 0.365206 |
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Target: 5'- gGGucacCCGAaGGAUuuuucCCCUUGCCGCCu -3' miRNA: 3'- -CCu---GGCUaUCUGcau--GGGAAUGGUGG- -5' |
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| 6160 | 5' | -50.6 | NC_001837.1 | + | 3550 | 0.77 | 0.094133 |
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Target: 5'- uGGACCuaGUGGuacuCGUACCCaagACCACCa -3' miRNA: 3'- -CCUGGc-UAUCu---GCAUGGGaa-UGGUGG- -5' |
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| 6160 | 5' | -50.6 | NC_001837.1 | + | 76 | 1.16 | 0.000115 |
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Target: 5'- cGGACCGAUAGACGUACCCUUACCACCu -3' miRNA: 3'- -CCUGGCUAUCUGCAUGGGAAUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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