Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6163 | 3' | -50 | NC_001837.1 | + | 948 | 0.66 | 0.436964 |
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Target: 5'- aGUCGaaguuGCAGCGCaAAGAGCAGUuaagagccGGCAu -3' miRNA: 3'- -CGGU-----UGUUGUGgUUCUCGUCA--------UCGUu -5' |
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| 6163 | 3' | -50 | NC_001837.1 | + | 3203 | 0.66 | 0.402592 |
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Target: 5'- gGCCAGCGGC----AGAGCGGUuGCGGg -3' miRNA: 3'- -CGGUUGUUGugguUCUCGUCAuCGUU- -5' |
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| 6163 | 3' | -50 | NC_001837.1 | + | 2687 | 0.69 | 0.292194 |
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Target: 5'- aCUAGCAugACUAGGGGCAGc-GCAc -3' miRNA: 3'- cGGUUGUugUGGUUCUCGUCauCGUu -5' |
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| 6163 | 3' | -50 | NC_001837.1 | + | 7512 | 0.69 | 0.283422 |
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Target: 5'- aGCCc-CAGCGCCAAGAG-GGUGGUc- -3' miRNA: 3'- -CGGuuGUUGUGGUUCUCgUCAUCGuu -5' |
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| 6163 | 3' | -50 | NC_001837.1 | + | 4861 | 0.7 | 0.227791 |
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Target: 5'- uGCCGgcgcuGCAGCAgCCAgcgcGGAGUAGUAGUg- -3' miRNA: 3'- -CGGU-----UGUUGU-GGU----UCUCGUCAUCGuu -5' |
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| 6163 | 3' | -50 | NC_001837.1 | + | 8021 | 0.71 | 0.20034 |
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Target: 5'- cGCCGuGCGGCugCAGGuuGgGGUAGCAAg -3' miRNA: 3'- -CGGU-UGUUGugGUUCu-CgUCAUCGUU- -5' |
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| 6163 | 3' | -50 | NC_001837.1 | + | 5918 | 0.73 | 0.153996 |
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Target: 5'- cCCAGCAuggugccucccACACCuAGGAGCAG-AGCAGc -3' miRNA: 3'- cGGUUGU-----------UGUGG-UUCUCGUCaUCGUU- -5' |
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| 6163 | 3' | -50 | NC_001837.1 | + | 6094 | 0.73 | 0.153996 |
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Target: 5'- uGCCuGGCcuCACCAGGGGCcucGGUGGCGGg -3' miRNA: 3'- -CGG-UUGuuGUGGUUCUCG---UCAUCGUU- -5' |
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| 6163 | 3' | -50 | NC_001837.1 | + | 5709 | 0.74 | 0.130188 |
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Target: 5'- gGCCAGCAA-GCCAGGGGCAuuGUcGGCGAg -3' miRNA: 3'- -CGGUUGUUgUGGUUCUCGU--CA-UCGUU- -5' |
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| 6163 | 3' | -50 | NC_001837.1 | + | 572 | 0.99 | 0.001388 |
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Target: 5'- gGCC-ACAACACCAAGAGCAGUAGCAAc -3' miRNA: 3'- -CGGuUGUUGUGGUUCUCGUCAUCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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