Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6163 | 5' | -53.7 | NC_001837.1 | + | 7070 | 0.67 | 0.222129 |
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Target: 5'- aGCAGC--CUugCAgGCUCuggaggAUGCUGCu -3' miRNA: 3'- aCGUCGauGGugGUgUGAG------UACGACG- -5' |
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| 6163 | 5' | -53.7 | NC_001837.1 | + | 413 | 0.67 | 0.222129 |
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Target: 5'- gGCAGCcaugggGCCACCcaggcgGCGC-CGUGCUacagGCg -3' miRNA: 3'- aCGUCGa-----UGGUGG------UGUGaGUACGA----CG- -5' |
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| 6163 | 5' | -53.7 | NC_001837.1 | + | 5869 | 0.69 | 0.165764 |
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Target: 5'- aGCGGCUGCUcuGCUccuaggugugggagGCAC-CAUGCUGg -3' miRNA: 3'- aCGUCGAUGG--UGG--------------UGUGaGUACGACg -5' |
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| 6163 | 5' | -53.7 | NC_001837.1 | + | 8438 | 0.69 | 0.15696 |
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Target: 5'- uUGCAGCUuCCACCuGCAUgUCAUGUUcGUc -3' miRNA: 3'- -ACGUCGAuGGUGG-UGUG-AGUACGA-CG- -5' |
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| 6163 | 5' | -53.7 | NC_001837.1 | + | 538 | 1.12 | 5.1e-05 |
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Target: 5'- uUGCAGCUACCACCACACUCAUGCUGCu -3' miRNA: 3'- -ACGUCGAUGGUGGUGUGAGUACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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