Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6169 | 3' | -54.7 | NC_001837.1 | + | 406 | 0.66 | 0.262054 |
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Target: 5'- gCCGAaaggCAGCCAUGGG-GC-CAC-CCAg -3' miRNA: 3'- -GGUUg---GUUGGUGCCCuCGuGUGuGGU- -5' |
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| 6169 | 3' | -54.7 | NC_001837.1 | + | 6958 | 0.68 | 0.177017 |
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Target: 5'- uCCuGCCGAggAgGGGGGCGCGCuCCAa -3' miRNA: 3'- -GGuUGGUUggUgCCCUCGUGUGuGGU- -5' |
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| 6169 | 3' | -54.7 | NC_001837.1 | + | 4140 | 0.69 | 0.151428 |
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Target: 5'- gCUucuCCAugUAUGGGcccauAGCGCGCACCGu -3' miRNA: 3'- -GGuu-GGUugGUGCCC-----UCGUGUGUGGU- -5' |
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| 6169 | 3' | -54.7 | NC_001837.1 | + | 2966 | 0.71 | 0.10221 |
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Target: 5'- aCCAGCCAcaguuugcacaaagGCCgGCGGGGGCGguUGCCu -3' miRNA: 3'- -GGUUGGU--------------UGG-UGCCCUCGUguGUGGu -5' |
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| 6169 | 3' | -54.7 | NC_001837.1 | + | 5631 | 0.72 | 0.099917 |
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Target: 5'- -aAGCUugGACCGCGGGGGUGCuggugcccccgGCACCAg -3' miRNA: 3'- ggUUGG--UUGGUGCCCUCGUG-----------UGUGGU- -5' |
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| 6169 | 3' | -54.7 | NC_001837.1 | + | 2549 | 1.11 | 0.000067 |
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Target: 5'- cCCAACCAACCACGGGAGCACACACCAg -3' miRNA: 3'- -GGUUGGUUGGUGCCCUCGUGUGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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