Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 617 | 3' | -51.3 | AC_000017.1 | + | 16431 | 1.16 | 0.000497 |
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Target: 5'- aCCCUGAGUCAUAGCACUAAUGGCCGCg -3' miRNA: 3'- -GGGACUCAGUAUCGUGAUUACCGGCG- -5' |
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| 617 | 3' | -51.3 | AC_000017.1 | + | 16006 | 0.68 | 0.702877 |
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Target: 5'- gCCCUGGGgcgc-GCACaaacgcGGCCGCa -3' miRNA: 3'- -GGGACUCaguauCGUGauua--CCGGCG- -5' |
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| 617 | 3' | -51.3 | AC_000017.1 | + | 25473 | 0.67 | 0.780245 |
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Target: 5'- gCCU---UCAacgAGCGCUccGUGGCCGCg -3' miRNA: 3'- gGGAcucAGUa--UCGUGAu-UACCGGCG- -5' |
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| 617 | 3' | -51.3 | AC_000017.1 | + | 317 | 0.66 | 0.790716 |
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Target: 5'- uUCUGuGuuacUCAUAGCGCgUAAUauuugucuagGGCCGCg -3' miRNA: 3'- gGGACuC----AGUAUCGUG-AUUA----------CCGGCG- -5' |
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| 617 | 3' | -51.3 | AC_000017.1 | + | 28000 | 0.66 | 0.790716 |
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Target: 5'- aCCgGAGccgcgggCAaAGCACUuGUGGCgGCg -3' miRNA: 3'- gGGaCUCa------GUaUCGUGAuUACCGgCG- -5' |
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| 617 | 3' | -51.3 | AC_000017.1 | + | 5336 | 0.66 | 0.79691 |
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Target: 5'- gUCUGGGUCAcggugaagggguGCGCUccgGGCUGCg -3' miRNA: 3'- gGGACUCAGUau----------CGUGAuuaCCGGCG- -5' |
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| 617 | 3' | -51.3 | AC_000017.1 | + | 34936 | 0.66 | 0.820963 |
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Target: 5'- uUCUUuuGUCAUGGUuccugGCggggaAGUGGCCGCg -3' miRNA: 3'- -GGGAcuCAGUAUCG-----UGa----UUACCGGCG- -5' |
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| 617 | 3' | -51.3 | AC_000017.1 | + | 15828 | 0.66 | 0.830616 |
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Target: 5'- gCCCUGGG-CAUAGU-CUc---GCCGCg -3' miRNA: 3'- -GGGACUCaGUAUCGuGAuuacCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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