Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 617 | 5' | -63.6 | AC_000017.1 | + | 26600 | 0.66 | 0.247805 |
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Target: 5'- aCGGCGGCgGCU----GCUUGGACUUa -3' miRNA: 3'- -GCCGCCGgCGAgcagCGGACCUGGAc -5' |
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| 617 | 5' | -63.6 | AC_000017.1 | + | 17354 | 0.66 | 0.247805 |
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Target: 5'- -cGCGGCCGCagCGgcCGCCUGcACCg- -3' miRNA: 3'- gcCGCCGGCGa-GCa-GCGGACcUGGac -5' |
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| 617 | 5' | -63.6 | AC_000017.1 | + | 6394 | 0.66 | 0.241634 |
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Target: 5'- aCGGUGGUgCGCUCGUCGggcaCCaGGugCa- -3' miRNA: 3'- -GCCGCCG-GCGAGCAGC----GGaCCugGac -5' |
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| 617 | 5' | -63.6 | AC_000017.1 | + | 23770 | 0.67 | 0.20726 |
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Target: 5'- uGGCGGgCGCUCGg-GCUUGGGagaUGg -3' miRNA: 3'- gCCGCCgGCGAGCagCGGACCUgg-AC- -5' |
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| 617 | 5' | -63.6 | AC_000017.1 | + | 6551 | 0.67 | 0.20726 |
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Target: 5'- gGGCGGCCGcCUC--UG-CUGGACCa- -3' miRNA: 3'- gCCGCCGGC-GAGcaGCgGACCUGGac -5' |
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| 617 | 5' | -63.6 | AC_000017.1 | + | 11546 | 0.68 | 0.172577 |
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Target: 5'- uCGGCGGCCGCcacgUGUgCGCgCgcgGGACUa- -3' miRNA: 3'- -GCCGCCGGCGa---GCA-GCG-Ga--CCUGGac -5' |
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| 617 | 5' | -63.6 | AC_000017.1 | + | 16397 | 1.08 | 0.000113 |
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Target: 5'- gCGGCGGCCGCUCGUCGCCUGGACCUGg -3' miRNA: 3'- -GCCGCCGGCGAGCAGCGGACCUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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