Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6172 | 3' | -57.7 | NC_001837.1 | + | 3180 | 0.67 | 0.117302 |
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Target: 5'- uACCCC---CCUGCGACguaggCGCUCgGCCa -3' miRNA: 3'- uUGGGGuaaGGGCGUUG-----GCGAGaCGG- -5' |
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| 6172 | 3' | -57.7 | NC_001837.1 | + | 9471 | 0.68 | 0.099936 |
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Target: 5'- -cCCCCAgaCuuGCGuCUGCUgCUGCCu -3' miRNA: 3'- uuGGGGUaaGggCGUuGGCGA-GACGG- -5' |
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| 6172 | 3' | -57.7 | NC_001837.1 | + | 230 | 0.69 | 0.090711 |
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Target: 5'- aGGCCCCAcUCCUGUgGACCGUgCcGCCa -3' miRNA: 3'- -UUGGGGUaAGGGCG-UUGGCGaGaCGG- -5' |
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| 6172 | 3' | -57.7 | NC_001837.1 | + | 8054 | 0.69 | 0.087819 |
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Target: 5'- uACCCCAa-CCUGCAGCCGCacgGCg -3' miRNA: 3'- uUGGGGUaaGGGCGUUGGCGagaCGg -5' |
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| 6172 | 3' | -57.7 | NC_001837.1 | + | 9032 | 0.7 | 0.074632 |
|
Target: 5'- --aCUCAUUCCCGCuGaaGCUCuUGCCu -3' miRNA: 3'- uugGGGUAAGGGCGuUggCGAG-ACGG- -5' |
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| 6172 | 3' | -57.7 | NC_001837.1 | + | 7111 | 0.7 | 0.074389 |
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Target: 5'- uGCCCCAUgugCCUGUcauaaugggugacGACUGUUCuUGCCg -3' miRNA: 3'- uUGGGGUAa--GGGCG-------------UUGGCGAG-ACGG- -5' |
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| 6172 | 3' | -57.7 | NC_001837.1 | + | 3245 | 1.1 | 0.00003 |
|
Target: 5'- aAACCCCAUUCCCGCAACCGCUCUGCCg -3' miRNA: 3'- -UUGGGGUAAGGGCGUUGGCGAGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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