Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6176 | 3' | -56.1 | NC_001837.1 | + | 1406 | 0.66 | 0.200188 |
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Target: 5'- gCCAuuGUaUgAugAGCUGCCCAG-GAGUg -3' miRNA: 3'- -GGU--CGgAgUugUCGACGGGUCuCUCG- -5' |
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| 6176 | 3' | -56.1 | NC_001837.1 | + | 4860 | 0.67 | 0.161234 |
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Target: 5'- gCCGGCgCUgCAGCAGCcaGCgCGGAGuAGUa -3' miRNA: 3'- -GGUCG-GA-GUUGUCGa-CGgGUCUC-UCG- -5' |
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| 6176 | 3' | -56.1 | NC_001837.1 | + | 2944 | 0.69 | 0.121241 |
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Target: 5'- gCCGGCgggGGCGGUUGCCUAuGGGAGUg -3' miRNA: 3'- -GGUCGgagUUGUCGACGGGU-CUCUCG- -5' |
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| 6176 | 3' | -56.1 | NC_001837.1 | + | 3793 | 0.69 | 0.110096 |
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Target: 5'- uCCAG-CUCAACAGuCUuCCC-GAGAGUg -3' miRNA: 3'- -GGUCgGAGUUGUC-GAcGGGuCUCUCG- -5' |
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| 6176 | 3' | -56.1 | NC_001837.1 | + | 747 | 0.73 | 0.053485 |
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Target: 5'- gCGGCCU----GGCUcccaGCCCGGAGAGCu -3' miRNA: 3'- gGUCGGAguugUCGA----CGGGUCUCUCG- -5' |
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| 6176 | 3' | -56.1 | NC_001837.1 | + | 4906 | 1.14 | 2.3e-05 |
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Target: 5'- aCCAGCCUCAACAGCUGCCCAGAGAGCu -3' miRNA: 3'- -GGUCGGAGUUGUCGACGGGUCUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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