Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
618 | 3' | -64.5 | AC_000017.1 | + | 13157 | 0.66 | 0.229769 |
Target: 5'- aGCCGCgCGCUGGGGCa---GGaGGACa -3' miRNA: 3'- aCGGUG-GCGGCUCCGcuggCC-CCUGc -5' |
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618 | 3' | -64.5 | AC_000017.1 | + | 27215 | 0.66 | 0.212931 |
Target: 5'- gUGCUGgCGCCGGGuGUGGCCGcuGGaGAUGa -3' miRNA: 3'- -ACGGUgGCGGCUC-CGCUGGC--CC-CUGC- -5' |
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618 | 3' | -64.5 | AC_000017.1 | + | 8984 | 0.66 | 0.202304 |
Target: 5'- cGCCACCGUgGA-GCGAgCCGGacGCGg -3' miRNA: 3'- aCGGUGGCGgCUcCGCU-GGCCccUGC- -5' |
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618 | 3' | -64.5 | AC_000017.1 | + | 12163 | 0.68 | 0.155853 |
Target: 5'- aGCCgACgCGCCcugGAGGCaGCUGGGGcCGg -3' miRNA: 3'- aCGG-UG-GCGG---CUCCGcUGGCCCCuGC- -5' |
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618 | 3' | -64.5 | AC_000017.1 | + | 26240 | 0.7 | 0.109955 |
Target: 5'- gGCUGCUGCUGAuagggcugcGGCGGCgGGGGAa- -3' miRNA: 3'- aCGGUGGCGGCU---------CCGCUGgCCCCUgc -5' |
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618 | 3' | -64.5 | AC_000017.1 | + | 11130 | 0.71 | 0.101325 |
Target: 5'- uUGCCGCUGCUGAGGag---GGGGGCGc -3' miRNA: 3'- -ACGGUGGCGGCUCCgcuggCCCCUGC- -5' |
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618 | 3' | -64.5 | AC_000017.1 | + | 23831 | 0.73 | 0.066986 |
Target: 5'- -uCCGCCGCCGAGGUugauGGCCGcGGGCu -3' miRNA: 3'- acGGUGGCGGCUCCG----CUGGCcCCUGc -5' |
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618 | 3' | -64.5 | AC_000017.1 | + | 23918 | 0.73 | 0.059915 |
Target: 5'- cGCCGCCucaGCCGcuuuuuuggGGGCGcGCgGGGGGCGg -3' miRNA: 3'- aCGGUGG---CGGC---------UCCGC-UGgCCCCUGC- -5' |
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618 | 3' | -64.5 | AC_000017.1 | + | 10376 | 0.74 | 0.055091 |
Target: 5'- gGCCAgCguagggugGCCGGGGCu-CCGGGGGCGa -3' miRNA: 3'- aCGGUgG--------CGGCUCCGcuGGCCCCUGC- -5' |
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618 | 3' | -64.5 | AC_000017.1 | + | 16284 | 0.76 | 0.035101 |
Target: 5'- gGCCGCCGCCGcgcguuGGGCGgcaguGCCGGGucGGCGg -3' miRNA: 3'- aCGGUGGCGGC------UCCGC-----UGGCCC--CUGC- -5' |
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618 | 3' | -64.5 | AC_000017.1 | + | 17322 | 1.07 | 0.000119 |
Target: 5'- cUGCCACCGCCGAGGCGACCGGGGACGu -3' miRNA: 3'- -ACGGUGGCGGCUCCGCUGGCCCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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