Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6180 | 5' | -63.1 | NC_001837.1 | + | 448 | 0.66 | 0.076596 |
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Target: 5'- cGCCGCCuggguGGCCCCaUGGcUGccuuucGGCCAu -3' miRNA: 3'- -CGGUGGcu---CCGGGG-ACC-ACu-----CCGGUc -5' |
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| 6180 | 5' | -63.1 | NC_001837.1 | + | 2845 | 0.66 | 0.071724 |
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Target: 5'- uGCCACCGuGGGUcaagagaCCCaUGGUGAcaagGGCCc- -3' miRNA: 3'- -CGGUGGC-UCCG-------GGG-ACCACU----CCGGuc -5' |
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| 6180 | 5' | -63.1 | NC_001837.1 | + | 5668 | 0.66 | 0.069729 |
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Target: 5'- aGCCcucGCCGAcaauGCCCCUGGcuUGcuGGCCAc -3' miRNA: 3'- -CGG---UGGCUc---CGGGGACC--ACu-CCGGUc -5' |
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| 6180 | 5' | -63.1 | NC_001837.1 | + | 539 | 0.68 | 0.048353 |
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Target: 5'- uGCUACUGcucuugguguuguuGGCCCCUGcG-GGGGCCAu -3' miRNA: 3'- -CGGUGGCu-------------CCGGGGAC-CaCUCCGGUc -5' |
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| 6180 | 5' | -63.1 | NC_001837.1 | + | 4412 | 0.75 | 0.012401 |
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Target: 5'- gGCCAUUGGGGCCCCcGGaGGGGUCGc -3' miRNA: 3'- -CGGUGGCUCCGGGGaCCaCUCCGGUc -5' |
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| 6180 | 5' | -63.1 | NC_001837.1 | + | 6132 | 1.1 | 0.000008 |
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Target: 5'- cGCCACCGAGGCCCCUGGUGAGGCCAGg -3' miRNA: 3'- -CGGUGGCUCCGGGGACCACUCCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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