Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6182 | 5' | -55.9 | NC_001837.1 | + | 3948 | 0.67 | 0.162194 |
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Target: 5'- -cCCUAgGGA---GGGUCUCCCAAGGc -3' miRNA: 3'- ccGGAUgUCUaacCCCGGAGGGUUCU- -5' |
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| 6182 | 5' | -55.9 | NC_001837.1 | + | 5926 | 0.68 | 0.152341 |
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Target: 5'- aGGCCUGuccCAGcaUGGuGCCUCCCAcaccuaGGAg -3' miRNA: 3'- -CCGGAU---GUCuaACCcCGGAGGGU------UCU- -5' |
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| 6182 | 5' | -55.9 | NC_001837.1 | + | 201 | 0.73 | 0.059506 |
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Target: 5'- cGGUCcACAGGagUGGGGCCUCCgGuguGAa -3' miRNA: 3'- -CCGGaUGUCUa-ACCCCGGAGGgUu--CU- -5' |
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| 6182 | 5' | -55.9 | NC_001837.1 | + | 7822 | 0.74 | 0.052105 |
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Target: 5'- aGGUCUGguGAUUGGGGgagguaugacCCUCCCAc-- -3' miRNA: 3'- -CCGGAUguCUAACCCC----------GGAGGGUucu -5' |
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| 6182 | 5' | -55.9 | NC_001837.1 | + | 404 | 0.74 | 0.04715 |
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Target: 5'- uGGCCgaaagGCAGccaUGGGGCCaCCCAGGc -3' miRNA: 3'- -CCGGa----UGUCua-ACCCCGGaGGGUUCu -5' |
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| 6182 | 5' | -55.9 | NC_001837.1 | + | 6857 | 1.11 | 0.000042 |
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Target: 5'- uGGCCUACAGAUUGGGGCCUCCCAAGAg -3' miRNA: 3'- -CCGGAUGUCUAACCCCGGAGGGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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