miRNA: 3'- -UCG-GACGU--UCCGACGAag-------GCguCGGa -5'
miRNA: 3'- -UCGGacgUUCCGACGaaggCGUCGGA- -5'
miRNA: 3'- -UCGGACGUUCCGACGAAGGCGUCGGA- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6183 | 3' | -59.5 | NC_001837.1 | + | 1254 | 0.66 | 0.10727 |
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Target: 5'- aAGCuCUGCAguGGGUUGCUguggcgugaCGguGCCc -3' miRNA: 3'- -UCG-GACGU--UCCGACGAag-------GCguCGGa -5' |
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| 6183 | 3' | -59.5 | NC_001837.1 | + | 7052 | 0.69 | 0.053992 |
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Target: 5'- uAGCCaa-GAGGCUGCggaaGCAGCCUu -3' miRNA: 3'- -UCGGacgUUCCGACGaaggCGUCGGA- -5' |
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| 6183 | 3' | -59.5 | NC_001837.1 | + | 7095 | 1.07 | 0.000027 |
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Target: 5'- gAGCCUGCAAGGCUGCUUCCGCAGCCUc -3' miRNA: 3'- -UCGGACGUUCCGACGAAGGCGUCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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