Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6183 | 5' | -52.1 | NC_001837.1 | + | 183 | 0.66 | 0.380054 |
Target: 5'- aGGGAaGCGGUcaccgggauaaGCcuGCCCUAUAGCg -3' miRNA: 3'- cCUCUaCGUCA-----------CGucUGGGAUAUCGg -5' |
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6183 | 5' | -52.1 | NC_001837.1 | + | 45 | 0.66 | 0.380054 |
Target: 5'- uGAGGUGguGggGCGGuuCCg--GGCCu -3' miRNA: 3'- cCUCUACguCa-CGUCugGGauaUCGG- -5' |
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6183 | 5' | -52.1 | NC_001837.1 | + | 3209 | 0.66 | 0.359661 |
Target: 5'- cGGcAGA-GCGGuUGCGGgaaugggguuuuGCCCUAgAGCCc -3' miRNA: 3'- -CC-UCUaCGUC-ACGUC------------UGGGAUaUCGG- -5' |
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6183 | 5' | -52.1 | NC_001837.1 | + | 5529 | 0.67 | 0.312096 |
Target: 5'- uGGAGAcUG-AGUGUggcugGGGCCCcAUGGCUg -3' miRNA: 3'- -CCUCU-ACgUCACG-----UCUGGGaUAUCGG- -5' |
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6183 | 5' | -52.1 | NC_001837.1 | + | 7516 | 0.68 | 0.253767 |
Target: 5'- cGAacUGCAG-GCGGACUCUcUAGCUg -3' miRNA: 3'- cCUcuACGUCaCGUCUGGGAuAUCGG- -5' |
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6183 | 5' | -52.1 | NC_001837.1 | + | 7060 | 1.14 | 0.000063 |
Target: 5'- gGGAGAUGCAGUGCAGACCCUAUAGCCu -3' miRNA: 3'- -CCUCUACGUCACGUCUGGGAUAUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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