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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6187 | 5' | -53.9 | NC_001837.1 | + | 450 | 0.68 | 0.223493 |
Target: 5'- ---gGCGCCG-CCugGGUGGCcCCAUGGc -3' miRNA: 3'- guagUGCGGUaGG--CCACUGuGGUACC- -5' |
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6187 | 5' | -53.9 | NC_001837.1 | + | 9019 | 0.71 | 0.13779 |
Target: 5'- aCAUCACcaacUCAUCCGGUGAUgaaccacuucgguauGCCAUGa -3' miRNA: 3'- -GUAGUGc---GGUAGGCCACUG---------------UGGUACc -5' |
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6187 | 5' | -53.9 | NC_001837.1 | + | 8531 | 1.12 | 0.000076 |
Target: 5'- aCAUCACGCCAUCCGGUGACACCAUGGg -3' miRNA: 3'- -GUAGUGCGGUAGGCCACUGUGGUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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