Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 619 | 3' | -50 | AC_000017.1 | + | 16067 | 0.66 | 0.845419 |
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Target: 5'- --cGGCCGcguuuguGCGCGCcCCAGGGgcgcggUAGUg -3' miRNA: 3'- uuaCUGGU-------UGCGCGaGGUUCCa-----AUUA- -5' |
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| 619 | 3' | -50 | AC_000017.1 | + | 27782 | 0.66 | 0.826937 |
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Target: 5'- cAAUGccuCCGgaGCGCgGCUCCGAGGaUGAg -3' miRNA: 3'- -UUACu--GGU--UGCG-CGAGGUUCCaAUUa -5' |
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| 619 | 3' | -50 | AC_000017.1 | + | 6322 | 0.69 | 0.657615 |
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Target: 5'- --cGAUCGGCGCGCUCCuuGGc---- -3' miRNA: 3'- uuaCUGGUUGCGCGAGGuuCCaauua -5' |
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| 619 | 3' | -50 | AC_000017.1 | + | 25894 | 0.69 | 0.657615 |
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Target: 5'- -cUGACgAAaagucCGCgGCUCCGGGGUUGAa -3' miRNA: 3'- uuACUGgUU-----GCG-CGAGGUUCCAAUUa -5' |
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| 619 | 3' | -50 | AC_000017.1 | + | 10375 | 0.69 | 0.633473 |
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Target: 5'- --gGGCCAGCGUaggguggccgggGCUCCGGGGgcGAg -3' miRNA: 3'- uuaCUGGUUGCG------------CGAGGUUCCaaUUa -5' |
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| 619 | 3' | -50 | AC_000017.1 | + | 23822 | 0.7 | 0.585272 |
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Target: 5'- -uUGGCCAAaucCGCcgCCGAGGUUGAUg -3' miRNA: 3'- uuACUGGUUgc-GCGa-GGUUCCAAUUA- -5' |
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| 619 | 3' | -50 | AC_000017.1 | + | 23864 | 0.72 | 0.469407 |
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Target: 5'- -uUGGCCAACGCGC-CCAAGa----- -3' miRNA: 3'- uuACUGGUUGCGCGaGGUUCcaauua -5' |
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| 619 | 3' | -50 | AC_000017.1 | + | 20489 | 1.04 | 0.003199 |
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Target: 5'- cAAUGACCAACGCGCUCCAAGGUUAAUg -3' miRNA: 3'- -UUACUGGUUGCGCGAGGUUCCAAUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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