Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6191 | 5' | -60.2 | NC_001837.1 | + | 207 | 0.66 | 0.103188 |
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Target: 5'- --aCAGGAguG-GG-GCCUCCGGUGUg -3' miRNA: 3'- cugGUUCUguCuCCgCGGGGGCCACG- -5' |
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| 6191 | 5' | -60.2 | NC_001837.1 | + | 9116 | 0.66 | 0.099999 |
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Target: 5'- aGACCAGGAUGGAGGCngggGCCgCUC--UGCg -3' miRNA: 3'- -CUGGUUCUGUCUCCG----CGG-GGGccACG- -5' |
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| 6191 | 5' | -60.2 | NC_001837.1 | + | 604 | 0.66 | 0.099999 |
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Target: 5'- uGCCGGGACGGAaaaaGGaugGCCCCCGcagggGCc -3' miRNA: 3'- cUGGUUCUGUCU----CCg--CGGGGGCca---CG- -5' |
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| 6191 | 5' | -60.2 | NC_001837.1 | + | 5637 | 0.68 | 0.070564 |
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Target: 5'- gGACCGcGGgGGugcuGGUGCCCCCGGcaccaGCc -3' miRNA: 3'- -CUGGUuCUgUCu---CCGCGGGGGCCa----CG- -5' |
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| 6191 | 5' | -60.2 | NC_001837.1 | + | 6056 | 0.71 | 0.039503 |
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Target: 5'- --gCAAGGCAGAGGCcaaggaCgCCUGGUGCa -3' miRNA: 3'- cugGUUCUGUCUCCGc-----GgGGGCCACG- -5' |
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| 6191 | 5' | -60.2 | NC_001837.1 | + | 3947 | 1.1 | 0.000018 |
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Target: 5'- aGACCAAGACAGAGGCGCCCCCGGUGCc -3' miRNA: 3'- -CUGGUUCUGUCUCCGCGGGGGCCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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