Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6197 | 3' | -61.8 | NC_001844.1 | + | 143682 | 0.66 | 0.610546 |
Target: 5'- --cGCGCCCUCCCUgGCcggagGCCCGu -3' miRNA: 3'- guaUGCGGGAGGGGgUGagg--CGGGUg -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 115490 | 0.66 | 0.610546 |
Target: 5'- --aGCGagaucCCCUCUCCCGC-CUGCCCu- -3' miRNA: 3'- guaUGC-----GGGAGGGGGUGaGGCGGGug -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 113936 | 0.67 | 0.590789 |
Target: 5'- aCAUGCGggccaCCCUCaCCCCuCgggUCGCCUGCg -3' miRNA: 3'- -GUAUGC-----GGGAG-GGGGuGa--GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 117246 | 0.67 | 0.590789 |
Target: 5'- ---cCGCCUUCCCCgGCccCCGagcCCCGCg -3' miRNA: 3'- guauGCGGGAGGGGgUGa-GGC---GGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 117177 | 0.68 | 0.503946 |
Target: 5'- gCAU-CGCCCgcucauaaCCCCGCUCCgggGCUCGCc -3' miRNA: 3'- -GUAuGCGGGag------GGGGUGAGG---CGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 140482 | 0.69 | 0.475272 |
Target: 5'- --cAUGgCCUCUCCCagcuggggugguaGCUCCGcCCCACu -3' miRNA: 3'- guaUGCgGGAGGGGG-------------UGAGGC-GGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 145314 | 0.7 | 0.414589 |
Target: 5'- ---cCGCCCacuaacCCgCCCACUaacCCGCCCACu -3' miRNA: 3'- guauGCGGGa-----GG-GGGUGA---GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 145458 | 0.7 | 0.414589 |
Target: 5'- ---cCGCCCacuaacCCgCCCACUaacCCGCCCACu -3' miRNA: 3'- guauGCGGGa-----GG-GGGUGA---GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 145422 | 0.7 | 0.414589 |
Target: 5'- ---cCGCCCacuaacCCgCCCACUaacCCGCCCACu -3' miRNA: 3'- guauGCGGGa-----GG-GGGUGA---GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 145386 | 0.7 | 0.414589 |
Target: 5'- ---cCGCCCacuaacCCgCCCACUaacCCGCCCACu -3' miRNA: 3'- guauGCGGGa-----GG-GGGUGA---GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 145350 | 0.7 | 0.414589 |
Target: 5'- ---cCGCCCacuaacCCgCCCACUaacCCGCCCACu -3' miRNA: 3'- guauGCGGGa-----GG-GGGUGA---GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 145278 | 0.7 | 0.414589 |
Target: 5'- ---cCGCCCacuaacCCgCCCACUaacCCGCCCACu -3' miRNA: 3'- guauGCGGGa-----GG-GGGUGA---GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 145242 | 0.7 | 0.414589 |
Target: 5'- ---cCGCCCacuaacCCgCCCACUaacCCGCCCACu -3' miRNA: 3'- guauGCGGGa-----GG-GGGUGA---GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 145206 | 0.7 | 0.414589 |
Target: 5'- ---cCGCCCacuaacCCgCCCACUaacCCGCCCACu -3' miRNA: 3'- guauGCGGGa-----GG-GGGUGA---GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 145170 | 0.7 | 0.414589 |
Target: 5'- ---cCGCCCacuaacCCgCCCACUaacCCGCCCACu -3' miRNA: 3'- guauGCGGGa-----GG-GGGUGA---GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 70712 | 0.71 | 0.368988 |
Target: 5'- --gACGCCCaUCUCCGCguuaaaggugcgugCCGCCCGCu -3' miRNA: 3'- guaUGCGGGaGGGGGUGa-------------GGCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 139973 | 0.71 | 0.365865 |
Target: 5'- --aACGCCUUCCCCUAgCUCUaCCCAa -3' miRNA: 3'- guaUGCGGGAGGGGGU-GAGGcGGGUg -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 119348 | 0.71 | 0.350525 |
Target: 5'- aGUGCGCCC-CCCCCACa-CGCaCACc -3' miRNA: 3'- gUAUGCGGGaGGGGGUGagGCGgGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 20236 | 0.73 | 0.256167 |
Target: 5'- -cUACGCCgC-CCgCCCACUCaGCCCACu -3' miRNA: 3'- guAUGCGG-GaGG-GGGUGAGgCGGGUG- -5' |
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6197 | 3' | -61.8 | NC_001844.1 | + | 114574 | 0.75 | 0.197492 |
Target: 5'- --cACGCCaC-CCCCCggggACUCUGCCCGCg -3' miRNA: 3'- guaUGCGG-GaGGGGG----UGAGGCGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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