miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
6198 5' -56.7 NC_001844.1 + 89311 0.66 0.808359
Target:  5'- aUGCGGGGGcaguUGUGUCCUgauuggAACGauuugucCCCAu -3'
miRNA:   3'- -ACGUCCCC----ACACGGGAa-----UUGU-------GGGUu -5'
6198 5' -56.7 NC_001844.1 + 117297 0.68 0.742831
Target:  5'- aUGUuuGGGGUGgacgacgcGCCCUUGGcCACCCc- -3'
miRNA:   3'- -ACGu-CCCCACa-------CGGGAAUU-GUGGGuu -5'
6198 5' -56.7 NC_001844.1 + 64495 0.68 0.73286
Target:  5'- aGCGuaGGUGUGgUCUUGACACCCc- -3'
miRNA:   3'- aCGUccCCACACgGGAAUUGUGGGuu -5'
6198 5' -56.7 NC_001844.1 + 2590 1.05 0.003016
Target:  5'- cUGCAGGGGUGUGCCCUUAACACCCAAc -3'
miRNA:   3'- -ACGUCCCCACACGGGAAUUGUGGGUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.