miRNA: 3'- ugUAGUCGuuGCACCACAG-CGGCGac -5'
miRNA: 3'- ugUAGUCGuuGCACCACAG-CGGCGac -5'
miRNA: 3'- ugUAGUCGuuGCACCACAG-CGGCGac -5'
miRNA: 3'- ugUAGUCGuuGCACCACAG----------CGGCGAc -5'
miRNA: 3'- -UGUAGUcGUUGCACCACAGCGGCGAC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6199 | 5' | -55.1 | NC_001844.1 | + | 128327 | 0.66 | 0.928127 |
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Target: 5'- -uGUCAGC--UGUGGUGUCaGCUGUg- -3' miRNA: 3'- ugUAGUCGuuGCACCACAG-CGGCGac -5' |
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| 6199 | 5' | -55.1 | NC_001844.1 | + | 128351 | 0.66 | 0.928127 |
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Target: 5'- -uGUCAGC--UGUGGUGUCaGCUGUg- -3' miRNA: 3'- ugUAGUCGuuGCACCACAG-CGGCGac -5' |
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| 6199 | 5' | -55.1 | NC_001844.1 | + | 128375 | 0.66 | 0.928127 |
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Target: 5'- -uGUCAGC--UGUGGUGUCaGCUGUg- -3' miRNA: 3'- ugUAGUCGuuGCACCACAG-CGGCGac -5' |
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| 6199 | 5' | -55.1 | NC_001844.1 | + | 128303 | 0.69 | 0.803837 |
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Target: 5'- -uGUCAGC--UGUGGUGUCagcuguggugGCCGCUa -3' miRNA: 3'- ugUAGUCGuuGCACCACAG----------CGGCGAc -5' |
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| 6199 | 5' | -55.1 | NC_001844.1 | + | 3888 | 0.94 | 0.029069 |
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Target: 5'- gACAUCA-CAACGUGGUGUCGCCGCUGc -3' miRNA: 3'- -UGUAGUcGUUGCACCACAGCGGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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