Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6200 | 5' | -52 | NC_001844.1 | + | 120903 | 0.66 | 0.982051 |
Target: 5'- gGCgccCGCGGc-GCUUUUGAGCAGCg -3' miRNA: 3'- gCGau-GUGUCauCGGAGAUUCGUCGg -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 140390 | 0.66 | 0.977499 |
Target: 5'- uGCccGCAgAGUGauGCCUC-AAGCGGCa -3' miRNA: 3'- gCGa-UGUgUCAU--CGGAGaUUCGUCGg -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 116489 | 0.67 | 0.954886 |
Target: 5'- uGCguugGC-CGG-GGCCUCUAAGCGcaagcgcuGCCa -3' miRNA: 3'- gCGa---UGuGUCaUCGGAGAUUCGU--------CGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 13912 | 0.68 | 0.946301 |
Target: 5'- gGCUccGCGCAGUuGCCgCUuAGCGcuGCCg -3' miRNA: 3'- gCGA--UGUGUCAuCGGaGAuUCGU--CGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 122001 | 0.68 | 0.946301 |
Target: 5'- gCGCcACcCAGUAGCgCUCcGAGuCGGCUg -3' miRNA: 3'- -GCGaUGuGUCAUCG-GAGaUUC-GUCGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 68444 | 0.68 | 0.936704 |
Target: 5'- gGCUAa--AGUGGCCgacgCccuGGCAGCCc -3' miRNA: 3'- gCGAUgugUCAUCGGa---Gau-UCGUCGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 65099 | 0.68 | 0.936197 |
Target: 5'- aGCUACccaucaaGCGGUGGagcaCUCUAAGgcuguaCAGCCu -3' miRNA: 3'- gCGAUG-------UGUCAUCg---GAGAUUC------GUCGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 69704 | 0.69 | 0.91381 |
Target: 5'- cCGCUgGCGCuucGUggAGCUUCUcccguggguacgcAAGCAGCCa -3' miRNA: 3'- -GCGA-UGUGu--CA--UCGGAGA-------------UUCGUCGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 59846 | 0.69 | 0.901735 |
Target: 5'- uGCUuuCAgGGcAGCUUCUGcGGCAGCCc -3' miRNA: 3'- gCGAu-GUgUCaUCGGAGAU-UCGUCGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 115756 | 0.69 | 0.901735 |
Target: 5'- aGCUacccggcccaaGCACAGgcuGCCUCUcaaCAGCCg -3' miRNA: 3'- gCGA-----------UGUGUCau-CGGAGAuucGUCGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 74497 | 0.7 | 0.888044 |
Target: 5'- gGCgGCACAGUcAGCCUgUcgcaugcugggAAGCGGCUu -3' miRNA: 3'- gCGaUGUGUCA-UCGGAgA-----------UUCGUCGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 136097 | 0.72 | 0.787187 |
Target: 5'- gCGCUACugGGUGGCgCUgCUGgacauggcGGgGGCCg -3' miRNA: 3'- -GCGAUGugUCAUCG-GA-GAU--------UCgUCGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 64421 | 0.72 | 0.767843 |
Target: 5'- cCGC--CGCGGUAGCCgau--GCAGCCa -3' miRNA: 3'- -GCGauGUGUCAUCGGagauuCGUCGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 115912 | 0.73 | 0.737873 |
Target: 5'- cCGC-ACGCGGcAGCCUC--GGUGGCCa -3' miRNA: 3'- -GCGaUGUGUCaUCGGAGauUCGUCGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 144513 | 0.73 | 0.727671 |
Target: 5'- gGCcGCGCGGUGG-CUCUGGGCugcGCCc -3' miRNA: 3'- gCGaUGUGUCAUCgGAGAUUCGu--CGG- -5' |
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6200 | 5' | -52 | NC_001844.1 | + | 13865 | 1.04 | 0.012984 |
Target: 5'- gCGC-ACACAGUAGCCUCUAAGCAGCCg -3' miRNA: 3'- -GCGaUGUGUCAUCGGAGAUUCGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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