Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6202 | 3' | -53.5 | NC_001844.1 | + | 23943 | 0.66 | 0.957808 |
Target: 5'- -cCGAGAGG-GUGGcuuGCuaCAUCAAGg -3' miRNA: 3'- auGCUUUCCgCACCu--CGcgGUAGUUC- -5' |
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6202 | 3' | -53.5 | NC_001844.1 | + | 113525 | 0.7 | 0.80522 |
Target: 5'- cUGCGGAcGGCGcGGAGCcgggGCCGUgGAGc -3' miRNA: 3'- -AUGCUUuCCGCaCCUCG----CGGUAgUUC- -5' |
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6202 | 3' | -53.5 | NC_001844.1 | + | 85536 | 0.71 | 0.767405 |
Target: 5'- cGCGGccacAGGCgGUGGAGUGUCAcCAAGg -3' miRNA: 3'- aUGCUu---UCCG-CACCUCGCGGUaGUUC- -5' |
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6202 | 3' | -53.5 | NC_001844.1 | + | 26417 | 1.07 | 0.005714 |
Target: 5'- gUACGAAAGGCGUGGAGCGCCAUCAAGu -3' miRNA: 3'- -AUGCUUUCCGCACCUCGCGGUAGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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