Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6204 | 5' | -55.8 | NC_001844.1 | + | 13537 | 0.66 | 0.881089 |
Target: 5'- -uCUGgaaaGCCCAGCucGGCuuguAGCCGCUg -3' miRNA: 3'- guGACa---CGGGUUG--UCGuu--UCGGCGGa -5' |
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6204 | 5' | -55.8 | NC_001844.1 | + | 32586 | 0.66 | 0.873775 |
Target: 5'- cCGC-GUGCCCcg-AGCAGAGCaCGUCa -3' miRNA: 3'- -GUGaCACGGGuugUCGUUUCG-GCGGa -5' |
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6204 | 5' | -55.8 | NC_001844.1 | + | 104752 | 0.67 | 0.82014 |
Target: 5'- gACUcUGaCCCAACGGCGAucagucuguauccccAGCCGCa- -3' miRNA: 3'- gUGAcAC-GGGUUGUCGUU---------------UCGGCGga -5' |
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6204 | 5' | -55.8 | NC_001844.1 | + | 113771 | 0.68 | 0.798591 |
Target: 5'- aGCUGUGUCgGGCGGCcAAcGUgGCCUa -3' miRNA: 3'- gUGACACGGgUUGUCGuUU-CGgCGGA- -5' |
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6204 | 5' | -55.8 | NC_001844.1 | + | 143727 | 0.69 | 0.741046 |
Target: 5'- -----aGCCCGGCGGCGGAGCgGUCc -3' miRNA: 3'- gugacaCGGGUUGUCGUUUCGgCGGa -5' |
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6204 | 5' | -55.8 | NC_001844.1 | + | 115457 | 0.69 | 0.740051 |
Target: 5'- gCGCUGUGCuCCAACcccgcugGGCucgagacuGCUGCCUg -3' miRNA: 3'- -GUGACACG-GGUUG-------UCGuuu-----CGGCGGA- -5' |
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6204 | 5' | -55.8 | NC_001844.1 | + | 107596 | 0.7 | 0.690273 |
Target: 5'- gACUGccaauaCCCAcCAGCAAAGCCaGCCa -3' miRNA: 3'- gUGACac----GGGUuGUCGUUUCGG-CGGa -5' |
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6204 | 5' | -55.8 | NC_001844.1 | + | 142977 | 0.7 | 0.690273 |
Target: 5'- uGCUGcUGCCCAAC-GUc--GCCGCCg -3' miRNA: 3'- gUGAC-ACGGGUUGuCGuuuCGGCGGa -5' |
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6204 | 5' | -55.8 | NC_001844.1 | + | 73656 | 0.71 | 0.596447 |
Target: 5'- -----cGCCCAGCAGCccucGCCGCCg -3' miRNA: 3'- gugacaCGGGUUGUCGuuu-CGGCGGa -5' |
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6204 | 5' | -55.8 | NC_001844.1 | + | 73745 | 0.71 | 0.596447 |
Target: 5'- -----cGCCCAGCAGCccucGCCGCCg -3' miRNA: 3'- gugacaCGGGUUGUCGuuu-CGGCGGa -5' |
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6204 | 5' | -55.8 | NC_001844.1 | + | 40847 | 1.08 | 0.002667 |
Target: 5'- cCACUGUGCCCAACAGCAAAGCCGCCUg -3' miRNA: 3'- -GUGACACGGGUUGUCGUUUCGGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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