Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6205 | 3' | -52.6 | NC_001844.1 | + | 67305 | 0.66 | 0.976337 |
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Target: 5'- aGCCCUUUguuUGGaCGCcCAAA-CUGGa -3' miRNA: 3'- aCGGGAGAac-ACC-GCGuGUUUaGACC- -5' |
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| 6205 | 3' | -52.6 | NC_001844.1 | + | 82506 | 0.66 | 0.976337 |
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Target: 5'- aGCCCcaUCUUGcggcUGGgGUACGGGcugCUGGu -3' miRNA: 3'- aCGGG--AGAAC----ACCgCGUGUUUa--GACC- -5' |
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| 6205 | 3' | -52.6 | NC_001844.1 | + | 88056 | 0.68 | 0.918265 |
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Target: 5'- cGCCaacUUUGUGGCGCGCuucAGUCgccUGGu -3' miRNA: 3'- aCGGga-GAACACCGCGUGu--UUAG---ACC- -5' |
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| 6205 | 3' | -52.6 | NC_001844.1 | + | 141668 | 0.68 | 0.918265 |
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Target: 5'- gGCCCcggUUGUGGCG-GCG-GUCUGGg -3' miRNA: 3'- aCGGGag-AACACCGCgUGUuUAGACC- -5' |
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| 6205 | 3' | -52.6 | NC_001844.1 | + | 82462 | 0.7 | 0.847267 |
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Target: 5'- gGCCaacCUCgcgUGUGGCGCuACcAAUCUGa -3' miRNA: 3'- aCGG---GAGa--ACACCGCG-UGuUUAGACc -5' |
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| 6205 | 3' | -52.6 | NC_001844.1 | + | 41911 | 1.1 | 0.004502 |
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Target: 5'- cUGCCCUCUUGUGGCGCACAAAUCUGGc -3' miRNA: 3'- -ACGGGAGAACACCGCGUGUUUAGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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