Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6207 | 3' | -51.3 | NC_001844.1 | + | 121838 | 0.69 | 0.94391 |
Target: 5'- cUCAGcgUgGGUcUCCACCGGCgcggcgccuaGGUCUa -3' miRNA: 3'- -AGUCuaAgUUA-AGGUGGCCG----------CCAGAg -5' |
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6207 | 3' | -51.3 | NC_001844.1 | + | 95469 | 0.7 | 0.904981 |
Target: 5'- gUAGAUUCAGUUCUAuaGGCaGGUC-Ca -3' miRNA: 3'- aGUCUAAGUUAAGGUggCCG-CCAGaG- -5' |
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6207 | 3' | -51.3 | NC_001844.1 | + | 59475 | 0.73 | 0.801539 |
Target: 5'- cUCAGcgUCuauagggCCACCGuaGGUCUCa -3' miRNA: 3'- -AGUCuaAGuuaa---GGUGGCcgCCAGAG- -5' |
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6207 | 3' | -51.3 | NC_001844.1 | + | 51012 | 1.1 | 0.006495 |
Target: 5'- aUCAGAUUCAAUUCCACCGGCGGUCUCc -3' miRNA: 3'- -AGUCUAAGUUAAGGUGGCCGCCAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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