Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6207 | 5' | -57.4 | NC_001844.1 | + | 133858 | 0.66 | 0.792097 |
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Target: 5'- uUAGCAG-CG-CGGCACAGGUGCa--- -3' miRNA: 3'- -GUCGUCgGCgGUCGUGUCUACGguuu -5' |
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| 6207 | 5' | -57.4 | NC_001844.1 | + | 143347 | 0.66 | 0.792097 |
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Target: 5'- gAGCucccccGCCGCCGGCucCAGggGCuCGGAg -3' miRNA: 3'- gUCGu-----CGGCGGUCGu-GUCuaCG-GUUU- -5' |
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| 6207 | 5' | -57.4 | NC_001844.1 | + | 115643 | 0.66 | 0.782712 |
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Target: 5'- aCGGCGGCCGCgGGUcCuGGUaCCAGAa -3' miRNA: 3'- -GUCGUCGGCGgUCGuGuCUAcGGUUU- -5' |
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| 6207 | 5' | -57.4 | NC_001844.1 | + | 46236 | 0.66 | 0.773184 |
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Target: 5'- aCAGUuccaAGCCGUCGGCGuCAGcaGCCGAu -3' miRNA: 3'- -GUCG----UCGGCGGUCGU-GUCuaCGGUUu -5' |
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| 6207 | 5' | -57.4 | NC_001844.1 | + | 57088 | 0.67 | 0.723759 |
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Target: 5'- gAGCAGgUGCCGGCAU-GA-GCCAGc -3' miRNA: 3'- gUCGUCgGCGGUCGUGuCUaCGGUUu -5' |
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| 6207 | 5' | -57.4 | NC_001844.1 | + | 78931 | 0.68 | 0.661822 |
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Target: 5'- uCGGCGGUccaacgcgggcgCGCUGGCaucaGCAGAUGCCAu- -3' miRNA: 3'- -GUCGUCG------------GCGGUCG----UGUCUACGGUuu -5' |
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| 6207 | 5' | -57.4 | NC_001844.1 | + | 68536 | 0.69 | 0.619899 |
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Target: 5'- gCGGCAGCCGCUGcCAUAGAcacGCUAGAa -3' miRNA: 3'- -GUCGUCGGCGGUcGUGUCUa--CGGUUU- -5' |
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| 6207 | 5' | -57.4 | NC_001844.1 | + | 50978 | 1.06 | 0.00229 |
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Target: 5'- gCAGCAGCCGCCAGCACAGAUGCCAAAa -3' miRNA: 3'- -GUCGUCGGCGGUCGUGUCUACGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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