Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6208 | 5' | -56.2 | NC_001844.1 | + | 41157 | 0.66 | 0.892803 |
Target: 5'- --aCCCCCAUugACGCUUgAAGGgugCGc -3' miRNA: 3'- guaGGGGGUGugUGCGGGgUUUCa--GU- -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 79474 | 0.67 | 0.84861 |
Target: 5'- aGUCUucugagagCCUGCACAUGCUCCAggAGGUCGc -3' miRNA: 3'- gUAGG--------GGGUGUGUGCGGGGU--UUCAGU- -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 143462 | 0.67 | 0.840513 |
Target: 5'- --gCCCCUcgggcgGCAUggGCCCCAAAGcUCGa -3' miRNA: 3'- guaGGGGG------UGUGugCGGGGUUUC-AGU- -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 143344 | 0.67 | 0.815102 |
Target: 5'- -cUCCCCCGC-CGCcgGCUCCAGGGg-- -3' miRNA: 3'- guAGGGGGUGuGUG--CGGGGUUUCagu -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 104054 | 0.68 | 0.806285 |
Target: 5'- --gCCCCCGCGCAUGCUCCc------ -3' miRNA: 3'- guaGGGGGUGUGUGCGGGGuuucagu -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 142906 | 0.68 | 0.797307 |
Target: 5'- -uUCCCCCA-ACAUGCCCCc-GGcCAg -3' miRNA: 3'- guAGGGGGUgUGUGCGGGGuuUCaGU- -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 56381 | 0.68 | 0.778909 |
Target: 5'- aGUCUaCUCGCGUACGCCCCAAGGg-- -3' miRNA: 3'- gUAGG-GGGUGUGUGCGGGGUUUCagu -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 56177 | 0.68 | 0.778909 |
Target: 5'- cCGUCCCCCugGCGCugaguGCgCCCAGAa--- -3' miRNA: 3'- -GUAGGGGGugUGUG-----CG-GGGUUUcagu -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 96683 | 0.68 | 0.768559 |
Target: 5'- -uUCCCCCAugggguuuagucuCACAgGgCgCCAAAGUCAa -3' miRNA: 3'- guAGGGGGU-------------GUGUgCgG-GGUUUCAGU- -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 113992 | 0.69 | 0.759979 |
Target: 5'- gGUCUCCCuagaGgACugGCCCCAGgacGGUCc -3' miRNA: 3'- gUAGGGGG----UgUGugCGGGGUU---UCAGu -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 113034 | 0.71 | 0.639387 |
Target: 5'- cCAUCCCCaa-ACGCGCCCCcu-GUUg -3' miRNA: 3'- -GUAGGGGgugUGUGCGGGGuuuCAGu -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 114813 | 0.71 | 0.608515 |
Target: 5'- cCGUCCCgUcaACGgGCGCCCCAGGGgCAa -3' miRNA: 3'- -GUAGGGgG--UGUgUGCGGGGUUUCaGU- -5' |
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6208 | 5' | -56.2 | NC_001844.1 | + | 58746 | 1.08 | 0.002776 |
Target: 5'- gCAUCCCCCACACACGCCCCAAAGUCAa -3' miRNA: 3'- -GUAGGGGGUGUGUGCGGGGUUUCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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