Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6209 | 5' | -59.4 | NC_001844.1 | + | 39711 | 0.66 | 0.732754 |
Target: 5'- aUGUGcGCGG-GCUACCugggccacgcucaauGCCuuGGCGUCUAa -3' miRNA: 3'- -ACGC-CGCCgCGAUGG---------------UGG--UCGUAGAU- -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 101190 | 0.66 | 0.728827 |
Target: 5'- cUGUGGUugaacuGGCGaggugUACCGCCAGCGUg-- -3' miRNA: 3'- -ACGCCG------CCGCg----AUGGUGGUCGUAgau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 50994 | 0.66 | 0.698991 |
Target: 5'- gGCGGUcuccccuugGGCaGCaGCCGCCAGCAc--- -3' miRNA: 3'- aCGCCG---------CCG-CGaUGGUGGUCGUagau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 144217 | 0.66 | 0.698991 |
Target: 5'- cGCGGCugagguGGCGCUcCCgggGCCAGCGa--- -3' miRNA: 3'- aCGCCG------CCGCGAuGG---UGGUCGUagau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 102762 | 0.66 | 0.688922 |
Target: 5'- cUGCGGCGacuuauaggcaGCGUUuaacauCCACCAGCAg--- -3' miRNA: 3'- -ACGCCGC-----------CGCGAu-----GGUGGUCGUagau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 117638 | 0.67 | 0.638043 |
Target: 5'- gUGgGGCGGaGCUACCACCccAGCu---- -3' miRNA: 3'- -ACgCCGCCgCGAUGGUGG--UCGuagau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 136546 | 0.67 | 0.638043 |
Target: 5'- gGCGGUgaGGgGCUGuaccCCACCAGCAc--- -3' miRNA: 3'- aCGCCG--CCgCGAU----GGUGGUCGUagau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 143722 | 0.67 | 0.62782 |
Target: 5'- gGCGGCGGaGCgguCCGCUGGCAg--- -3' miRNA: 3'- aCGCCGCCgCGau-GGUGGUCGUagau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 134537 | 0.68 | 0.617601 |
Target: 5'- -aCGGCGGUGCUA--ACCGGCucuUCUGc -3' miRNA: 3'- acGCCGCCGCGAUggUGGUCGu--AGAU- -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 137936 | 0.69 | 0.546772 |
Target: 5'- aGCgGGgGGCGCcguCCACCAGCcccAUCa- -3' miRNA: 3'- aCG-CCgCCGCGau-GGUGGUCG---UAGau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 140840 | 0.69 | 0.536834 |
Target: 5'- gGCGGCGGgaUGaCUACCGCCGGgGUg-- -3' miRNA: 3'- aCGCCGCC--GC-GAUGGUGGUCgUAgau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 38939 | 0.69 | 0.507424 |
Target: 5'- -cUGGCGGCGCUAauuUCugCAGCAcgCUGu -3' miRNA: 3'- acGCCGCCGCGAU---GGugGUCGUa-GAU- -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 82453 | 0.7 | 0.469329 |
Target: 5'- cGCGuGUGGCGCUACCAaucugaaugaUGGCGUUUAc -3' miRNA: 3'- aCGC-CGCCGCGAUGGUg---------GUCGUAGAU- -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 122603 | 0.7 | 0.450836 |
Target: 5'- gGCGGCGaCGCgugGCCcucacggucGCCGGCGUCg- -3' miRNA: 3'- aCGCCGCcGCGa--UGG---------UGGUCGUAGau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 142206 | 0.71 | 0.395346 |
Target: 5'- aGCGGCGGCucauggccaccgagGCUGCCGCguGCGg--- -3' miRNA: 3'- aCGCCGCCG--------------CGAUGGUGguCGUagau -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 41513 | 0.72 | 0.356929 |
Target: 5'- gGUGGgGGCgGCUACCGgCAGCggCUAu -3' miRNA: 3'- aCGCCgCCG-CGAUGGUgGUCGuaGAU- -5' |
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6209 | 5' | -59.4 | NC_001844.1 | + | 66734 | 1.07 | 0.001523 |
Target: 5'- uUGCGGCGGCGCUACCACCAGCAUCUAg -3' miRNA: 3'- -ACGCCGCCGCGAUGGUGGUCGUAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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