Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 621 | 5' | -56.7 | AC_000017.1 | + | 10353 | 0.66 | 0.472189 |
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Target: 5'- gCGGCggCGGCuGGCGGuagaggGGCCAGc -3' miRNA: 3'- aGUUGgaGCCG-CCGCCuaaa--CCGGUU- -5' |
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| 621 | 5' | -56.7 | AC_000017.1 | + | 5120 | 0.67 | 0.441384 |
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Target: 5'- --cGCUgggCGGCGagcgccuucuaGCGGAUUUGGUCAGg -3' miRNA: 3'- aguUGGa--GCCGC-----------CGCCUAAACCGGUU- -5' |
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| 621 | 5' | -56.7 | AC_000017.1 | + | 8192 | 0.67 | 0.431371 |
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Target: 5'- ---cCCUCGcCuGGCGGGUUUGGCUg- -3' miRNA: 3'- aguuGGAGCcG-CCGCCUAAACCGGuu -5' |
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| 621 | 5' | -56.7 | AC_000017.1 | + | 17271 | 0.68 | 0.34763 |
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Target: 5'- --cGCCUCGGCGGUGGcagauGCCGc -3' miRNA: 3'- aguUGGAGCCGCCGCCuaaacCGGUu -5' |
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| 621 | 5' | -56.7 | AC_000017.1 | + | 17582 | 0.69 | 0.314325 |
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Target: 5'- gCGACggCGGCGGCGGGUUccagUGGUgAu -3' miRNA: 3'- aGUUGgaGCCGCCGCCUAA----ACCGgUu -5' |
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| 621 | 5' | -56.7 | AC_000017.1 | + | 3855 | 0.69 | 0.314325 |
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Target: 5'- gCGGCUgaagCGGCGGCGGA---GGCUg- -3' miRNA: 3'- aGUUGGa---GCCGCCGCCUaaaCCGGuu -5' |
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| 621 | 5' | -56.7 | AC_000017.1 | + | 18254 | 0.7 | 0.283491 |
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Target: 5'- -uGGCCUCuggcauuagcggGGUGGUGGAccUGGCCAAc -3' miRNA: 3'- agUUGGAG------------CCGCCGCCUaaACCGGUU- -5' |
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| 621 | 5' | -56.7 | AC_000017.1 | + | 10007 | 0.76 | 0.100512 |
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Target: 5'- aUCGcuGCggCGGCGGCGGAgUUUGGCCGu -3' miRNA: 3'- -AGU--UGgaGCCGCCGCCU-AAACCGGUu -5' |
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| 621 | 5' | -56.7 | AC_000017.1 | + | 23883 | 1.06 | 0.000546 |
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Target: 5'- aUCAACCUCGGCGGCGGAUUUGGCCAAc -3' miRNA: 3'- -AGUUGGAGCCGCCGCCUAAACCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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