Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6211 | 3' | -59.5 | NC_001844.1 | + | 138190 | 0.66 | 0.781198 |
Target: 5'- gGUGGUCgUGGggGugGUCGUGgGgGUg -3' miRNA: 3'- -CGUCGGgACCuuCugCGGCGCgCaCG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 138226 | 0.66 | 0.781198 |
Target: 5'- gGUGGUCgUGGggGugGUCGUGgGgGUg -3' miRNA: 3'- -CGUCGGgACCuuCugCGGCGCgCaCG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 138262 | 0.66 | 0.781198 |
Target: 5'- gGUGGUCgUGGggGugGUCGUGgGgGUg -3' miRNA: 3'- -CGUCGGgACCuuCugCGGCGCgCaCG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 138298 | 0.66 | 0.781198 |
Target: 5'- gGUGGUCgUGGggGugGUCGUGgGgGUg -3' miRNA: 3'- -CGUCGGgACCuuCugCGGCGCgCaCG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 138154 | 0.66 | 0.781198 |
Target: 5'- gGUGGUCgUGGggGugGUCGUGgGgGUg -3' miRNA: 3'- -CGUCGGgACCuuCugCGGCGCgCaCG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 144542 | 0.66 | 0.772094 |
Target: 5'- cGCAGCCaUGccccAAGGCcccaucgguugGCCGCGCGgugGCu -3' miRNA: 3'- -CGUCGGgACc---UUCUG-----------CGGCGCGCa--CG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 99664 | 0.66 | 0.772094 |
Target: 5'- uGCuaaCCCUGGAcggccaagaAGACGCCGagguuuCGUGUg -3' miRNA: 3'- -CGuc-GGGACCU---------UCUGCGGCgc----GCACG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 141074 | 0.66 | 0.76287 |
Target: 5'- uCGGCCCcGGGAGACcCCGgGCaggucucgGUGUc -3' miRNA: 3'- cGUCGGGaCCUUCUGcGGCgCG--------CACG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 34345 | 0.66 | 0.753536 |
Target: 5'- aGUAGCUCauUGucuAG-UGCUGCGCGUGCu -3' miRNA: 3'- -CGUCGGG--ACcu-UCuGCGGCGCGCACG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 115667 | 0.66 | 0.744099 |
Target: 5'- cGCGGCCCUcGGccacggcgcAAGACggcgGCCGCGgGUc- -3' miRNA: 3'- -CGUCGGGA-CC---------UUCUG----CGGCGCgCAcg -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 142586 | 0.66 | 0.744099 |
Target: 5'- -aAGCCCggGGAcagcGGACGCucCGCcGCGgagGCg -3' miRNA: 3'- cgUCGGGa-CCU----UCUGCG--GCG-CGCa--CG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 113895 | 0.67 | 0.705503 |
Target: 5'- cGCuGGCCCcGGGAGcgccaccucaGCCGCGCG-GUc -3' miRNA: 3'- -CG-UCGGGaCCUUCug--------CGGCGCGCaCG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 122593 | 0.67 | 0.705503 |
Target: 5'- cGUGGCCCUcacGGuCGCCGgCGuCGUGCu -3' miRNA: 3'- -CGUCGGGAccuUCuGCGGC-GC-GCACG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 120924 | 0.67 | 0.705503 |
Target: 5'- gGC-GCCCgccuuuggGGAcaGGGCGCC-CGCG-GCg -3' miRNA: 3'- -CGuCGGGa-------CCU--UCUGCGGcGCGCaCG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 94007 | 0.67 | 0.675904 |
Target: 5'- -aGGCauuaaCUGGAAGAUGUgGCuuGUGCa -3' miRNA: 3'- cgUCGg----GACCUUCUGCGgCGcgCACG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 121696 | 0.68 | 0.66994 |
Target: 5'- cGCGGCCagauuucgGGggGAgGCCccgcuccucuccugaGCGCGUcaGCg -3' miRNA: 3'- -CGUCGGga------CCuuCUgCGG---------------CGCGCA--CG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 70431 | 0.68 | 0.625991 |
Target: 5'- cGC-GCuCUUGGAAGGCGUCcaucugugugGCGCGaUGCu -3' miRNA: 3'- -CGuCG-GGACCUUCUGCGG----------CGCGC-ACG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 65002 | 0.7 | 0.546696 |
Target: 5'- aGCAGCCaaacGGuAGACGCUaGCGCGUc- -3' miRNA: 3'- -CGUCGGga--CCuUCUGCGG-CGCGCAcg -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 15329 | 0.7 | 0.536972 |
Target: 5'- cGCgAGaCCUUGGugcuuGugGCUGCGCGgggGCa -3' miRNA: 3'- -CG-UC-GGGACCuu---CugCGGCGCGCa--CG- -5' |
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6211 | 3' | -59.5 | NC_001844.1 | + | 133444 | 0.7 | 0.51771 |
Target: 5'- cGUAGCCCUgacGGGAGAUGUaaaGCagcagGCGUGUu -3' miRNA: 3'- -CGUCGGGA---CCUUCUGCGg--CG-----CGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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