Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6211 | 5' | -56.4 | NC_001844.1 | + | 101001 | 0.66 | 0.845619 |
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Target: 5'- -aGCGGAuuUGCGGCGaaguguguaacugccACGCCCUAc--- -3' miRNA: 3'- caCGUCU--ACGCCGU---------------UGCGGGGUucau -5' |
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| 6211 | 5' | -56.4 | NC_001844.1 | + | 77322 | 0.67 | 0.779937 |
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Target: 5'- -gGCGGAUgaaacugcugGCGGUAGCGCUCCGGu-- -3' miRNA: 3'- caCGUCUA----------CGCCGUUGCGGGGUUcau -5' |
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| 6211 | 5' | -56.4 | NC_001844.1 | + | 12508 | 0.68 | 0.731051 |
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Target: 5'- uUGUAGugaGCGGCAGCGCCUCugcuuugucuGGUGu -3' miRNA: 3'- cACGUCua-CGCCGUUGCGGGGu---------UCAU- -5' |
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| 6211 | 5' | -56.4 | NC_001844.1 | + | 142209 | 0.68 | 0.720966 |
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Target: 5'- -cGUAGcgGCGGCucAugGCCaCCGAGg- -3' miRNA: 3'- caCGUCuaCGCCG--UugCGG-GGUUCau -5' |
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| 6211 | 5' | -56.4 | NC_001844.1 | + | 122060 | 0.71 | 0.565367 |
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Target: 5'- -cGCGGcgGCGGCGGCGUgUCGGGg- -3' miRNA: 3'- caCGUCuaCGCCGUUGCGgGGUUCau -5' |
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| 6211 | 5' | -56.4 | NC_001844.1 | + | 68389 | 1.07 | 0.002667 |
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Target: 5'- aGUGCAGAUGCGGCAACGCCCCAAGUAa -3' miRNA: 3'- -CACGUCUACGCCGUUGCGGGGUUCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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