Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6212 | 5' | -56.5 | NC_001844.1 | + | 114849 | 0.66 | 0.892814 |
Target: 5'- aGGgGGCGG-ACCGGCAcccuACGCCu-- -3' miRNA: 3'- gCCgCUGCCgUGGUCGUu---UGUGGuga -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 106558 | 0.66 | 0.892814 |
Target: 5'- aGGCcacCGGCgcugACCAGCucgacauGCACCACc -3' miRNA: 3'- gCCGcu-GCCG----UGGUCGuu-----UGUGGUGa -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 121821 | 0.66 | 0.879019 |
Target: 5'- cCGGCG-CGGCGCCuaggucuaccaGGUAccucccCACCGCg -3' miRNA: 3'- -GCCGCuGCCGUGG-----------UCGUuu----GUGGUGa -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 34294 | 0.66 | 0.879019 |
Target: 5'- gGGCG-CGGUGCCAuGCu-ACGCCGu- -3' miRNA: 3'- gCCGCuGCCGUGGU-CGuuUGUGGUga -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 140913 | 0.66 | 0.864342 |
Target: 5'- gGGCGAugcCGGCACUgcugcucuGGCcGGAgACCGCUu -3' miRNA: 3'- gCCGCU---GCCGUGG--------UCG-UUUgUGGUGA- -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 64671 | 0.67 | 0.84077 |
Target: 5'- gGGCGAaaUGGCAgUucuuGGCAAGCGCgACa -3' miRNA: 3'- gCCGCU--GCCGUgG----UCGUUUGUGgUGa -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 66732 | 0.67 | 0.84077 |
Target: 5'- gCGGCGGCGcuacCACCAGCAucuagguuAC-CCAUUa -3' miRNA: 3'- -GCCGCUGCc---GUGGUCGUu-------UGuGGUGA- -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 144596 | 0.67 | 0.832523 |
Target: 5'- uCGGCGggcuccACGGCcCCGGCuccGCGCCGu- -3' miRNA: 3'- -GCCGC------UGCCGuGGUCGuu-UGUGGUga -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 143724 | 0.67 | 0.816355 |
Target: 5'- cCGGCGGCGGagcgguccgcuggcaGCCAGCucuccaAAGCGCgCGCc -3' miRNA: 3'- -GCCGCUGCCg--------------UGGUCG------UUUGUG-GUGa -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 136545 | 0.68 | 0.797784 |
Target: 5'- gCGGUGAgGGgcuguacccCACCAGCAcgGACACgGCc -3' miRNA: 3'- -GCCGCUgCC---------GUGGUCGU--UUGUGgUGa -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 8206 | 0.68 | 0.77948 |
Target: 5'- uGcGCGACGaCACUGGCGucugccGCGCCGCUa -3' miRNA: 3'- gC-CGCUGCcGUGGUCGUu-----UGUGGUGA- -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 134758 | 0.68 | 0.770125 |
Target: 5'- gGGCGcACuGaGCACCAGUGAACACUg-- -3' miRNA: 3'- gCCGC-UG-C-CGUGGUCGUUUGUGGuga -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 45371 | 0.69 | 0.751053 |
Target: 5'- aCGuuGGCGGCAgCAGCAGAacgguCACUGCUg -3' miRNA: 3'- -GCcgCUGCCGUgGUCGUUU-----GUGGUGA- -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 41511 | 0.69 | 0.721682 |
Target: 5'- uGGgGGCGGCuACCGGCAGcgGCUAUUc -3' miRNA: 3'- gCCgCUGCCG-UGGUCGUUugUGGUGA- -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 12062 | 0.69 | 0.711725 |
Target: 5'- aGGCG-CGGUauGCCuGGCAAGCaacgGCCACg -3' miRNA: 3'- gCCGCuGCCG--UGG-UCGUUUG----UGGUGa -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 121267 | 0.69 | 0.7017 |
Target: 5'- aCGGCGAUaaaggGGCACCguugGGCGuACGCaCACg -3' miRNA: 3'- -GCCGCUG-----CCGUGG----UCGUuUGUG-GUGa -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 22397 | 0.71 | 0.589462 |
Target: 5'- uGGCGAaGGCACCAGUucuccagucACACCAa- -3' miRNA: 3'- gCCGCUgCCGUGGUCGuu-------UGUGGUga -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 144206 | 0.74 | 0.434481 |
Target: 5'- uGGCGcuccCGGgGCCAGCGAACGgCGCa -3' miRNA: 3'- gCCGCu---GCCgUGGUCGUUUGUgGUGa -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 78916 | 0.77 | 0.312894 |
Target: 5'- gGGCGcGCuGGCAUCAGCAGAUGCCAUc -3' miRNA: 3'- gCCGC-UG-CCGUGGUCGUUUGUGGUGa -5' |
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6212 | 5' | -56.5 | NC_001844.1 | + | 68935 | 0.93 | 0.030093 |
Target: 5'- uCGGCGACGGC-CCaAGCAAACACCACUa -3' miRNA: 3'- -GCCGCUGCCGuGG-UCGUUUGUGGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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