Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6213 | 5' | -58.1 | NC_001844.1 | + | 15936 | 0.66 | 0.856434 |
Target: 5'- cGGGUGGUgaauagcgcgCACCCaUGACUGguaaGCGCUg -3' miRNA: 3'- -CCCAUCGa---------GUGGG-GUUGGCag--CGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 44042 | 0.66 | 0.84876 |
Target: 5'- uGGUAGa----CCCAACCGcCGUGCUu -3' miRNA: 3'- cCCAUCgagugGGGUUGGCaGCGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 117291 | 0.66 | 0.844065 |
Target: 5'- gGGGUggacgacgcgcccuuGGC-CACCCCGGCgGUagucauccCGcCGCCu -3' miRNA: 3'- -CCCA---------------UCGaGUGGGGUUGgCA--------GC-GCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 136056 | 0.67 | 0.80771 |
Target: 5'- cGG-AGCUauuuCCCCGACaCGcCGcCGCCg -3' miRNA: 3'- cCCaUCGAgu--GGGGUUG-GCaGC-GCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 122040 | 0.67 | 0.789282 |
Target: 5'- gGGGaaaUAGCUCcggaucgGCCCCcGCCauGUCcagcaGCGCCa -3' miRNA: 3'- -CCC---AUCGAG-------UGGGGuUGG--CAG-----CGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 105613 | 0.67 | 0.772093 |
Target: 5'- ----cGCUCGUCUCAACgGUCGCGCg -3' miRNA: 3'- cccauCGAGUGGGGUUGgCAGCGCGg -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 38068 | 0.67 | 0.772093 |
Target: 5'- -cGUGGCUCGCgCCUcuCUGgugCGCGCUu -3' miRNA: 3'- ccCAUCGAGUG-GGGuuGGCa--GCGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 113741 | 0.68 | 0.753536 |
Target: 5'- cGGUGGa-CACCCgGGCCGgagaGCGCa -3' miRNA: 3'- cCCAUCgaGUGGGgUUGGCag--CGCGg -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 40666 | 0.68 | 0.752596 |
Target: 5'- uGGGUugccucuguugagGGCUCGCuUCUAGCCGcCGCaGCUu -3' miRNA: 3'- -CCCA-------------UCGAGUG-GGGUUGGCaGCG-CGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 11954 | 0.68 | 0.734569 |
Target: 5'- ---aGGCUgCAUgCCAAaaGUCGCGCCu -3' miRNA: 3'- cccaUCGA-GUGgGGUUggCAGCGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 143350 | 0.69 | 0.675903 |
Target: 5'- cGGG-AGCUC-CCCCGccGCCGgcUCcaggggcucggaGCGCCg -3' miRNA: 3'- -CCCaUCGAGuGGGGU--UGGC--AG------------CGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 117637 | 0.7 | 0.635994 |
Target: 5'- uGGGgcggAGCUacCACCCCAGCUGggaGagGCCa -3' miRNA: 3'- -CCCa---UCGA--GUGGGGUUGGCag-Cg-CGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 113886 | 0.7 | 0.615991 |
Target: 5'- cGGG-AGCgcCACCUCAGCCG-CGCGguCCu -3' miRNA: 3'- -CCCaUCGa-GUGGGGUUGGCaGCGC--GG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 116239 | 0.7 | 0.605006 |
Target: 5'- cGGGacgGGCUCugggaugACCCCGaaauaguucugGCCGccUCGCGCUa -3' miRNA: 3'- -CCCa--UCGAG-------UGGGGU-----------UGGC--AGCGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 116294 | 0.71 | 0.566306 |
Target: 5'- cGGGUcGgaCGCCCCAccACCGgggagggUGCGCUa -3' miRNA: 3'- -CCCAuCgaGUGGGGU--UGGCa------GCGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 142983 | 0.71 | 0.566306 |
Target: 5'- aGGGacuGCUgCugCCCAA-CGUCGcCGCCg -3' miRNA: 3'- -CCCau-CGA-GugGGGUUgGCAGC-GCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 144595 | 0.72 | 0.527308 |
Target: 5'- cGGcgGGCUCcacgGCCCCGGCUc-CGCGCCg -3' miRNA: 3'- cCCa-UCGAG----UGGGGUUGGcaGCGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 114275 | 0.72 | 0.498729 |
Target: 5'- uGGGUGGaCUCccaaccaCCCGACCcggcggcUCGCGCCg -3' miRNA: 3'- -CCCAUC-GAGug-----GGGUUGGc------AGCGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 137744 | 0.73 | 0.461748 |
Target: 5'- cGGG-AGCguucugCGCUCC-GCCGUgGCGCCc -3' miRNA: 3'- -CCCaUCGa-----GUGGGGuUGGCAgCGCGG- -5' |
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6213 | 5' | -58.1 | NC_001844.1 | + | 142334 | 0.74 | 0.39245 |
Target: 5'- cGGGUAGCUagccgugACCCCGGCCGcCGagugggaguccUGCCg -3' miRNA: 3'- -CCCAUCGAg------UGGGGUUGGCaGC-----------GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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