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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6217 | 5' | -59.9 | NC_001844.1 | + | 50271 | 0.67 | 0.666307 |
Target: 5'- gGCCAGGCGcGCCuuuugcaacucuauCUCGUCU-GGUu-- -3' miRNA: 3'- gCGGUCCGC-CGG--------------GAGCAGAgCCAuuu -5' |
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6217 | 5' | -59.9 | NC_001844.1 | + | 115800 | 0.69 | 0.510432 |
Target: 5'- aCGCCGGGCGGCaggaCUCccaCUCGGc--- -3' miRNA: 3'- -GCGGUCCGCCGg---GAGca-GAGCCauuu -5' |
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6217 | 5' | -59.9 | NC_001844.1 | + | 77995 | 1.06 | 0.001782 |
Target: 5'- aCGCCAGGCGGCCCUCGUCUCGGUAAAu -3' miRNA: 3'- -GCGGUCCGCCGGGAGCAGAGCCAUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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