Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6219 | 3' | -54.1 | NC_001844.1 | + | 55224 | 0.66 | 0.93736 |
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Target: 5'- gCUGAUGGCgCACCAaCAaAGCGUUg-- -3' miRNA: 3'- -GACUACCG-GUGGUcGUgUCGUAGaug -5' |
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| 6219 | 3' | -54.1 | NC_001844.1 | + | 61215 | 0.67 | 0.90919 |
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Target: 5'- aUGuaaauGCCGCUAGCGCAGCGcUgUGCg -3' miRNA: 3'- gACuac--CGGUGGUCGUGUCGU-AgAUG- -5' |
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| 6219 | 3' | -54.1 | NC_001844.1 | + | 115896 | 0.67 | 0.882118 |
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Target: 5'- -cGGUGGCCAUgAGC-C-GCcgCUACg -3' miRNA: 3'- gaCUACCGGUGgUCGuGuCGuaGAUG- -5' |
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| 6219 | 3' | -54.1 | NC_001844.1 | + | 114450 | 0.68 | 0.86714 |
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Target: 5'- ---cUGGCUACCAuCGCAGCccgCUGCa -3' miRNA: 3'- gacuACCGGUGGUcGUGUCGua-GAUG- -5' |
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| 6219 | 3' | -54.1 | NC_001844.1 | + | 61266 | 0.68 | 0.859309 |
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Target: 5'- uUGggGGCCACaaaUAGCACGGCAa---- -3' miRNA: 3'- gACuaCCGGUG---GUCGUGUCGUagaug -5' |
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| 6219 | 3' | -54.1 | NC_001844.1 | + | 121844 | 0.7 | 0.740492 |
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Target: 5'- gCUGAgcucagcgUGGgucuCCACCGGCGCGGCgccuagGUCUACc -3' miRNA: 3'- -GACU--------ACC----GGUGGUCGUGUCG------UAGAUG- -5' |
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| 6219 | 3' | -54.1 | NC_001844.1 | + | 117451 | 0.78 | 0.350446 |
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Target: 5'- --cAUGGCCAgCCAGCGCAGCGauuuugcuccggaccUCUACg -3' miRNA: 3'- gacUACCGGU-GGUCGUGUCGU---------------AGAUG- -5' |
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| 6219 | 3' | -54.1 | NC_001844.1 | + | 78794 | 1.11 | 0.002536 |
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Target: 5'- cCUGAUGGCCACCAGCACAGCAUCUACg -3' miRNA: 3'- -GACUACCGGUGGUCGUGUCGUAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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