Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6219 | 5' | -59 | NC_001844.1 | + | 38068 | 0.66 | 0.781159 |
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Target: 5'- -cGUGGCucGCGCCucUCUGGUGcGCGCUu -3' miRNA: 3'- gaCACCG--UGUGGcaAGGUCGC-CGCGA- -5' |
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| 6219 | 5' | -59 | NC_001844.1 | + | 34931 | 0.66 | 0.753161 |
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Target: 5'- ---cGGUugGCCGUUCCGGUaaaGGCauGCg -3' miRNA: 3'- gacaCCGugUGGCAAGGUCG---CCG--CGa -5' |
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| 6219 | 5' | -59 | NC_001844.1 | + | 102167 | 0.67 | 0.694589 |
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Target: 5'- --uUGGCAUcuuuucaaACCaUUCCAGCGGCGg- -3' miRNA: 3'- gacACCGUG--------UGGcAAGGUCGCCGCga -5' |
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| 6219 | 5' | -59 | NC_001844.1 | + | 115058 | 0.67 | 0.690598 |
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Target: 5'- uUGUGGCcCACUGUUCCcgggaagccucucGUGGUGCc -3' miRNA: 3'- gACACCGuGUGGCAAGGu------------CGCCGCGa -5' |
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| 6219 | 5' | -59 | NC_001844.1 | + | 144208 | 0.72 | 0.413754 |
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Target: 5'- -gGUGGCGCucCCGgggCCAGCgaacGGCGCa -3' miRNA: 3'- gaCACCGUGu-GGCaa-GGUCG----CCGCGa -5' |
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| 6219 | 5' | -59 | NC_001844.1 | + | 3614 | 0.77 | 0.203522 |
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Target: 5'- gUGUGGCGCACCGUUUaguGCGGCa-- -3' miRNA: 3'- gACACCGUGUGGCAAGgu-CGCCGcga -5' |
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| 6219 | 5' | -59 | NC_001844.1 | + | 78755 | 0.97 | 0.009307 |
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Target: 5'- gCUGUGGCACACCGUUCCAGCGGC-CUa -3' miRNA: 3'- -GACACCGUGUGGCAAGGUCGCCGcGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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