Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6220 | 5' | -55.9 | NC_001844.1 | + | 12906 | 0.66 | 0.878726 |
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Target: 5'- uCGCUuGCAUguGCGGcgGCUA-CGCg -3' miRNA: 3'- -GCGAcCGUAguCGUCuaCGGUaGCGa -5' |
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| 6220 | 5' | -55.9 | NC_001844.1 | + | 77912 | 0.66 | 0.878726 |
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Target: 5'- aGCUGGU--CGGCAG-UGCC-UCGUUc -3' miRNA: 3'- gCGACCGuaGUCGUCuACGGuAGCGA- -5' |
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| 6220 | 5' | -55.9 | NC_001844.1 | + | 141775 | 0.66 | 0.863771 |
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Target: 5'- cCGgaUGGUGUgAGCAGcgGCUcgCGCUg -3' miRNA: 3'- -GCg-ACCGUAgUCGUCuaCGGuaGCGA- -5' |
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| 6220 | 5' | -55.9 | NC_001844.1 | + | 137563 | 0.66 | 0.855965 |
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Target: 5'- uGUUGGCAggcccuuuugCAGCuGAUGCCGgaGCa -3' miRNA: 3'- gCGACCGUa---------GUCGuCUACGGUagCGa -5' |
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| 6220 | 5' | -55.9 | NC_001844.1 | + | 120150 | 0.67 | 0.80497 |
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Target: 5'- cCGCUGuGaCAUgCGGCAGGauCCGUCGCa -3' miRNA: 3'- -GCGAC-C-GUA-GUCGUCUacGGUAGCGa -5' |
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| 6220 | 5' | -55.9 | NC_001844.1 | + | 45371 | 0.7 | 0.677199 |
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Target: 5'- aCGUUGGCGgcagCAGCAGAacgGUCAcUGCUg -3' miRNA: 3'- -GCGACCGUa---GUCGUCUa--CGGUaGCGA- -5' |
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| 6220 | 5' | -55.9 | NC_001844.1 | + | 78912 | 1.07 | 0.003016 |
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Target: 5'- gCGCUGGCAUCAGCAGAUGCCAUCGCUc -3' miRNA: 3'- -GCGACCGUAGUCGUCUACGGUAGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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