Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6221 | 5' | -55.4 | NC_001844.1 | + | 111499 | 0.66 | 0.88814 |
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Target: 5'- cUUUGAGGGCCaauuuugggaUGCugAuagaggcgaUGCUCCGGa -3' miRNA: 3'- aAGACUCUCGG----------ACGugU---------ACGAGGUCc -5' |
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| 6221 | 5' | -55.4 | NC_001844.1 | + | 143350 | 0.66 | 0.866101 |
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Target: 5'- ---cGGGAGCUcccccGcCGCcgGCUCCAGGg -3' miRNA: 3'- aagaCUCUCGGa----C-GUGuaCGAGGUCC- -5' |
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| 6221 | 5' | -55.4 | NC_001844.1 | + | 123224 | 0.66 | 0.866101 |
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Target: 5'- --gUGAGAuGCCUgGC-CGUGUUCCAGu -3' miRNA: 3'- aagACUCU-CGGA-CGuGUACGAGGUCc -5' |
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| 6221 | 5' | -55.4 | NC_001844.1 | + | 23849 | 0.67 | 0.833646 |
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Target: 5'- uUUCUGuGAGCgCUGCACcgGaucCCAGa -3' miRNA: 3'- -AAGACuCUCG-GACGUGuaCga-GGUCc -5' |
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| 6221 | 5' | -55.4 | NC_001844.1 | + | 82208 | 0.68 | 0.769768 |
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Target: 5'- --gUGAGGGCCUGCACcgcacgaGCUaCCAGc -3' miRNA: 3'- aagACUCUCGGACGUGua-----CGA-GGUCc -5' |
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| 6221 | 5' | -55.4 | NC_001844.1 | + | 79471 | 1.08 | 0.002664 |
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Target: 5'- cUUCUGAGAGCCUGCACAUGCUCCAGGa -3' miRNA: 3'- -AAGACUCUCGGACGUGUACGAGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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