Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6225 | 5' | -52.1 | NC_001844.1 | + | 58820 | 0.66 | 0.988499 |
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Target: 5'- gGGUgaAACCguuuuugUCCGG-CGGGcgcagaacucgAGGCCGg -3' miRNA: 3'- -CCAa-UUGGaa-----AGGCCaGCCC-----------UUCGGC- -5' |
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| 6225 | 5' | -52.1 | NC_001844.1 | + | 73919 | 0.66 | 0.986974 |
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Target: 5'- ----cACCUUUCCuauuuaagCGGGGAGCCu -3' miRNA: 3'- ccaauUGGAAAGGcca-----GCCCUUCGGc -5' |
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| 6225 | 5' | -52.1 | NC_001844.1 | + | 120297 | 0.67 | 0.977877 |
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Target: 5'- cGGcaGGCCguaUUCCGGauggucagagccgcCGGGAAGCCu -3' miRNA: 3'- -CCaaUUGGa--AAGGCCa-------------GCCCUUCGGc -5' |
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| 6225 | 5' | -52.1 | NC_001844.1 | + | 44008 | 0.69 | 0.946134 |
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Target: 5'- uGGUUu-UUUUUCCGGcucuuuUUGGGGAGCUGg -3' miRNA: 3'- -CCAAuuGGAAAGGCC------AGCCCUUCGGC- -5' |
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| 6225 | 5' | -52.1 | NC_001844.1 | + | 143202 | 0.71 | 0.867656 |
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Target: 5'- aGGaUAGCCUgggaggcagCCGGUgGGGAgcgAGCCu -3' miRNA: 3'- -CCaAUUGGAaa-------GGCCAgCCCU---UCGGc -5' |
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| 6225 | 5' | -52.1 | NC_001844.1 | + | 110837 | 1.11 | 0.005305 |
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Target: 5'- aGGUUAACCUUUCCGGUCGGGAAGCCGu -3' miRNA: 3'- -CCAAUUGGAAAGGCCAGCCCUUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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