Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6228 | 5' | -54.5 | NC_001844.1 | + | 4414 | 0.66 | 0.943225 |
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Target: 5'- uCCGGACGCAcuuGgaCCc---GCUGGGCa -3' miRNA: 3'- -GGUCUGCGUu--CgaGGaucaCGACCUG- -5' |
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| 6228 | 5' | -54.5 | NC_001844.1 | + | 69852 | 0.66 | 0.943225 |
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Target: 5'- gCCAGuACGCcauAGGCUCUggaaaguaUAGaGUUGGACu -3' miRNA: 3'- -GGUC-UGCG---UUCGAGG--------AUCaCGACCUG- -5' |
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| 6228 | 5' | -54.5 | NC_001844.1 | + | 128663 | 0.66 | 0.922766 |
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Target: 5'- aCAGACGUuucGCUa--GGUGUUGGGCu -3' miRNA: 3'- gGUCUGCGuu-CGAggaUCACGACCUG- -5' |
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| 6228 | 5' | -54.5 | NC_001844.1 | + | 141485 | 0.67 | 0.884683 |
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Target: 5'- uCCAGGuacucuaaaaUGCGAGCUCCcGGgGCgagGGGCc -3' miRNA: 3'- -GGUCU----------GCGUUCGAGGaUCaCGa--CCUG- -5' |
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| 6228 | 5' | -54.5 | NC_001844.1 | + | 59870 | 0.68 | 0.862459 |
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Target: 5'- cCCGGAaaCGCGccuAGCUUCgAGUGCUuucaGGGCa -3' miRNA: 3'- -GGUCU--GCGU---UCGAGGaUCACGA----CCUG- -5' |
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| 6228 | 5' | -54.5 | NC_001844.1 | + | 114021 | 1.12 | 0.002127 |
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Target: 5'- gCCAGACGCAAGCUCCUAGUGCUGGACg -3' miRNA: 3'- -GGUCUGCGUUCGAGGAUCACGACCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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