Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6229 | 5' | -53.9 | NC_001844.1 | + | 141478 | 0.66 | 0.965356 |
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Target: 5'- aCUCUaaaauGCGAGCUCccggggcgAGGGGCCcgcccgGGUUa -3' miRNA: 3'- gGAGA-----UGCUUGAG--------UCCCUGGa-----CCAGc -5' |
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| 6229 | 5' | -53.9 | NC_001844.1 | + | 140878 | 0.67 | 0.941819 |
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Target: 5'- gCUUUGCcuucccgccgcgGGGCUCGGGGGCCgGGgaaggCGg -3' miRNA: 3'- gGAGAUG------------CUUGAGUCCCUGGaCCa----GC- -5' |
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| 6229 | 5' | -53.9 | NC_001844.1 | + | 143460 | 0.67 | 0.941356 |
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Target: 5'- cCCUCggGCGGcaugggccccaaaGCUCgAGGGGCCUGaGUa- -3' miRNA: 3'- -GGAGa-UGCU-------------UGAG-UCCCUGGAC-CAgc -5' |
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| 6229 | 5' | -53.9 | NC_001844.1 | + | 117122 | 0.69 | 0.897212 |
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Target: 5'- aCUCguccGCG-GCUaCAGGGGCC-GGUCGc -3' miRNA: 3'- gGAGa---UGCuUGA-GUCCCUGGaCCAGC- -5' |
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| 6229 | 5' | -53.9 | NC_001844.1 | + | 143762 | 0.72 | 0.757234 |
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Target: 5'- aCgUCUuCGGGgUCGGGGAUCUGGUUc -3' miRNA: 3'- -GgAGAuGCUUgAGUCCCUGGACCAGc -5' |
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| 6229 | 5' | -53.9 | NC_001844.1 | + | 115414 | 1.12 | 0.003503 |
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Target: 5'- cCCUCUACGAACUCAGGGACCUGGUCGa -3' miRNA: 3'- -GGAGAUGCUUGAGUCCCUGGACCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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