Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6240 | 3' | -52.2 | NC_001844.1 | + | 54377 | 0.66 | 0.978441 |
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Target: 5'- aAGCGCCGcccUGGGaaauaGCAAcgGAGGAa -3' miRNA: 3'- gUCGUGGUc--ACCCg----CGUUuuUUCCUc -5' |
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| 6240 | 3' | -52.2 | NC_001844.1 | + | 114423 | 0.66 | 0.97594 |
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Target: 5'- gGGCcuccgGCCAGggaGGGCGCGcgcuuuGGAGa -3' miRNA: 3'- gUCG-----UGGUCa--CCCGCGUuuuuu-CCUC- -5' |
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| 6240 | 3' | -52.2 | NC_001844.1 | + | 100403 | 0.66 | 0.967172 |
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Target: 5'- -uGCACCGcucauggGGGCGCcAAAGccGGAGa -3' miRNA: 3'- guCGUGGUca-----CCCGCG-UUUUuuCCUC- -5' |
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| 6240 | 3' | -52.2 | NC_001844.1 | + | 143189 | 0.67 | 0.963802 |
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Target: 5'- aGGCAgCCGGUGGGgaGCGAGccuucugccccGAGGGGc -3' miRNA: 3'- gUCGU-GGUCACCCg-CGUUU-----------UUUCCUc -5' |
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| 6240 | 3' | -52.2 | NC_001844.1 | + | 145075 | 0.68 | 0.94333 |
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Target: 5'- aAGCAaCAGgGGGCGCGuuuGGGGAu -3' miRNA: 3'- gUCGUgGUCaCCCGCGUuuuUUCCUc -5' |
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| 6240 | 3' | -52.2 | NC_001844.1 | + | 122112 | 0.68 | 0.94333 |
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Target: 5'- --cCGCCuGUGGGgGCAGGGGgagcGGGAGc -3' miRNA: 3'- gucGUGGuCACCCgCGUUUUU----UCCUC- -5' |
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| 6240 | 3' | -52.2 | NC_001844.1 | + | 142631 | 0.69 | 0.903945 |
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Target: 5'- aCAGCGCUucgGGaGGGCagGCGGGAGAGGGa -3' miRNA: 3'- -GUCGUGG---UCaCCCG--CGUUUUUUCCUc -5' |
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| 6240 | 3' | -52.2 | NC_001844.1 | + | 65436 | 0.71 | 0.826699 |
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Target: 5'- -cGCGcCCAGUGGaGCGUAGAGcGGGGc -3' miRNA: 3'- guCGU-GGUCACC-CGCGUUUUuUCCUc -5' |
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| 6240 | 3' | -52.2 | NC_001844.1 | + | 43638 | 0.74 | 0.6677 |
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Target: 5'- gCGGCGCCuuuuUGGGUGgCAAuAGAGGAGg -3' miRNA: 3'- -GUCGUGGuc--ACCCGC-GUUuUUUCCUC- -5' |
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| 6240 | 3' | -52.2 | NC_001844.1 | + | 121877 | 1.1 | 0.004834 |
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Target: 5'- gCAGCACCAGUGGGCGCAAAAAAGGAGg -3' miRNA: 3'- -GUCGUGGUCACCCGCGUUUUUUCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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