Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6242 | 5' | -60.4 | NC_001844.1 | + | 136304 | 0.66 | 0.694402 |
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Target: 5'- cCGCGCGguggGGaGGUACCuGguagaccuaggcgcCGCGCCGGu -3' miRNA: 3'- -GCGCGCaa--CC-CCGUGGuU--------------GCGCGGUU- -5' |
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| 6242 | 5' | -60.4 | NC_001844.1 | + | 101369 | 0.66 | 0.694402 |
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Target: 5'- aGCGUGuUUGGGGCuaauACCccuacagaggcuuaAGCGCGCUc- -3' miRNA: 3'- gCGCGC-AACCCCG----UGG--------------UUGCGCGGuu -5' |
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| 6242 | 5' | -60.4 | NC_001844.1 | + | 17378 | 0.69 | 0.508557 |
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Target: 5'- -uUGCGUUGGGGguCCAGgGCGUgGu -3' miRNA: 3'- gcGCGCAACCCCguGGUUgCGCGgUu -5' |
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| 6242 | 5' | -60.4 | NC_001844.1 | + | 105598 | 0.7 | 0.461791 |
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Target: 5'- uCGCGCGgcgaGGGGCGCaauuCGCGCa-- -3' miRNA: 3'- -GCGCGCaa--CCCCGUGguu-GCGCGguu -5' |
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| 6242 | 5' | -60.4 | NC_001844.1 | + | 129545 | 1.06 | 0.001626 |
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Target: 5'- uCGCGCGUUGGGGCACCAACGCGCCAAg -3' miRNA: 3'- -GCGCGCAACCCCGUGGUUGCGCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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