Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6244 | 5' | -56 | NC_001844.1 | + | 16075 | 0.66 | 0.858816 |
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Target: 5'- -cUAGCAAUCCCUCaaaCAcUGGGGgUGu -3' miRNA: 3'- cuGUUGUUAGGGGGg--GUuACCCCgAC- -5' |
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| 6244 | 5' | -56 | NC_001844.1 | + | 80993 | 0.67 | 0.842823 |
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Target: 5'- -cCAGCGcUCCUCCCUc-UGGGGUUGc -3' miRNA: 3'- cuGUUGUuAGGGGGGGuuACCCCGAC- -5' |
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| 6244 | 5' | -56 | NC_001844.1 | + | 140116 | 0.68 | 0.808542 |
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Target: 5'- uACAACGAUgggguuuguacCUCCCCCAAUgggaGGGGCc- -3' miRNA: 3'- cUGUUGUUA-----------GGGGGGGUUA----CCCCGac -5' |
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| 6244 | 5' | -56 | NC_001844.1 | + | 141825 | 0.68 | 0.79954 |
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Target: 5'- cAUAGCGcacCCUCCCCGgugGUGGGGCg- -3' miRNA: 3'- cUGUUGUua-GGGGGGGU---UACCCCGac -5' |
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| 6244 | 5' | -56 | NC_001844.1 | + | 116666 | 0.68 | 0.790383 |
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Target: 5'- -cCGGCGcUCCCCCCCGAguaacccGGGCg- -3' miRNA: 3'- cuGUUGUuAGGGGGGGUUac-----CCCGac -5' |
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| 6244 | 5' | -56 | NC_001844.1 | + | 142664 | 0.74 | 0.441545 |
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Target: 5'- aGGCAGCAGUCUCgagCCCAGcGGGGUUGg -3' miRNA: 3'- -CUGUUGUUAGGGg--GGGUUaCCCCGAC- -5' |
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| 6244 | 5' | -56 | NC_001844.1 | + | 135051 | 1.09 | 0.002525 |
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Target: 5'- gGACAACAAUCCCCCCCAAUGGGGCUGg -3' miRNA: 3'- -CUGUUGUUAGGGGGGGUUACCCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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