Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6248 | 5' | -58.4 | NC_001844.1 | + | 11676 | 0.66 | 0.774641 |
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Target: 5'- uGCucacuGACGUAGGUguaCGGGU-UGCCUGCu -3' miRNA: 3'- -CGu----CUGCGUCCG---GCUCAuACGGGCGu -5' |
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| 6248 | 5' | -58.4 | NC_001844.1 | + | 142859 | 0.68 | 0.677206 |
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Target: 5'- gGguGAUGgucCAGGCCGAGgcccggGCCCGg- -3' miRNA: 3'- -CguCUGC---GUCCGGCUCaua---CGGGCgu -5' |
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| 6248 | 5' | -58.4 | NC_001844.1 | + | 120942 | 0.69 | 0.636717 |
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Target: 5'- gGCGaGCGCuuuuGGCCGGGc--GCCCGCc -3' miRNA: 3'- -CGUcUGCGu---CCGGCUCauaCGGGCGu -5' |
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| 6248 | 5' | -58.4 | NC_001844.1 | + | 57102 | 0.69 | 0.616419 |
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Target: 5'- cGCcuGugG-AGGCCGAGcagGUGCCgGCAu -3' miRNA: 3'- -CGu-CugCgUCCGGCUCa--UACGGgCGU- -5' |
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| 6248 | 5' | -58.4 | NC_001844.1 | + | 97152 | 0.69 | 0.596168 |
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Target: 5'- aCAGAUGCGuuuaCGAGUGcUGCCCGCGg -3' miRNA: 3'- cGUCUGCGUccg-GCUCAU-ACGGGCGU- -5' |
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| 6248 | 5' | -58.4 | NC_001844.1 | + | 113524 | 0.7 | 0.546108 |
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Target: 5'- uGCGGacgGCGCGGaGCCGGGgccguggaGCCCGCc -3' miRNA: 3'- -CGUC---UGCGUC-CGGCUCaua-----CGGGCGu -5' |
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| 6248 | 5' | -58.4 | NC_001844.1 | + | 112471 | 0.7 | 0.546108 |
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Target: 5'- uCGGGCGCGGGCaaGGUAaacGCCCGUg -3' miRNA: 3'- cGUCUGCGUCCGgcUCAUa--CGGGCGu -5' |
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| 6248 | 5' | -58.4 | NC_001844.1 | + | 144181 | 0.72 | 0.424085 |
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Target: 5'- cGCAGGCGacccgaGGGgUGAGggugGCCCGCAu -3' miRNA: 3'- -CGUCUGCg-----UCCgGCUCaua-CGGGCGU- -5' |
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| 6248 | 5' | -58.4 | NC_001844.1 | + | 140409 | 1.1 | 0.001309 |
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Target: 5'- gGCAGACGCAGGCCGAGUAUGCCCGCAg -3' miRNA: 3'- -CGUCUGCGUCCGGCUCAUACGGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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