Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 625 | 3' | -58.5 | AC_000017.1 | + | 18889 | 0.66 | 0.427477 |
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Target: 5'- -cGGGCCagGACGccucggaguaCCugAGCCCCGGGc -3' miRNA: 3'- caUUCGG--CUGCa---------GGugUCGGGGCCUc -5' |
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| 625 | 3' | -58.5 | AC_000017.1 | + | 12161 | 0.66 | 0.408447 |
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Target: 5'- -cAAGCCGACGc-----GCCCUGGAGg -3' miRNA: 3'- caUUCGGCUGCagguguCGGGGCCUC- -5' |
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| 625 | 3' | -58.5 | AC_000017.1 | + | 6564 | 0.67 | 0.346192 |
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Target: 5'- -gGGGUCcGCGUCCACGGUaaagauCCCGGGc -3' miRNA: 3'- caUUCGGcUGCAGGUGUCG------GGGCCUc -5' |
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| 625 | 3' | -58.5 | AC_000017.1 | + | 10374 | 0.68 | 0.328889 |
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Target: 5'- -gGGGCCaGCGUaggguggCCGgGGCUCCGGGGg -3' miRNA: 3'- caUUCGGcUGCA-------GGUgUCGGGGCCUC- -5' |
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| 625 | 3' | -58.5 | AC_000017.1 | + | 24045 | 0.71 | 0.191134 |
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Target: 5'- --cGGCgCGACGUCCACcaaCCUGGAGg -3' miRNA: 3'- cauUCG-GCUGCAGGUGucgGGGCCUC- -5' |
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| 625 | 3' | -58.5 | AC_000017.1 | + | 25891 | 0.73 | 0.141141 |
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Target: 5'- ---cGCCuGACGaaaagUCCGCGGCUCCGGGGu -3' miRNA: 3'- cauuCGG-CUGC-----AGGUGUCGGGGCCUC- -5' |
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| 625 | 3' | -58.5 | AC_000017.1 | + | 25991 | 1.07 | 0.000362 |
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Target: 5'- gGUAAGCCGACGUCCACAGCCCCGGAGu -3' miRNA: 3'- -CAUUCGGCUGCAGGUGUCGGGGCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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